Detailed information of TA system TA160475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4389389..4390647
• Replicon: chromosome
• Accession: NZ_CP054793
• Organism: Pseudomonas aeruginosa strain SE5452

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HUK77_RS20140
• Protein ID: WP_003163133.1
• Coordinates: 4389389..4390144 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HUK77_RS20145
• Protein ID: WP_003086329.1
• Coordinates: 4390279..4390647 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK77_RS20130 (HUK77_20125)	4384561..4387104	+	2544	WP_003140632.1	acyl-homoserine-lactone acylase QuiP	-
HUK77_RS20135 (HUK77_20130)	4387259..4388620	-	1362	WP_079262974.1	DNA recombination protein RmuC	-
HUK77_RS20140 (HUK77_20135)	4389389..4390144	-	756	WP_003163133.1	RES family NAD+ phosphorylase	Toxin
HUK77_RS20145 (HUK77_20140)	4390279..4390647	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HUK77_RS20150 (HUK77_20145)	4390720..4392006	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
HUK77_RS20155 (HUK77_20150)	4392062..4393651	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
HUK77_RS20160 (HUK77_20155)	4393730..4394206	+	477	WP_003112531.1	hypothetical protein	-
HUK77_RS20165 (HUK77_20160)	4394209..4394484	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T160475 WP_003163133.1 NZ_CP054793:c4390144-4389389 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T160475 NZ_CP070521:8993-9307 [Morganella morganii]
ATGCAATTCACTGTATATAGTAATACAGGGAAAAGTGCCGTTTATCCCCTGCTGATTGATGTAACAAGCGATATTATCGG
CCAGTTAAACCGGAGGATTGTTATCCCCCTGTTACCTGTTGGAATGTACCCTAATGGTATGCGACCGGAGCGCCTGACTC
CGCTTATCAGGCTGGTTGATGATAATGAGTATGTAGTCATGACTCATGAAATGGCGAGTATTCCTGCTCGTTCTTTGGGG
GCTGCGTTCTGTGATGCTTCTTCTTATCGTAAGCAGATCAAGGATGCTATTGATTTTCTTCTCGATGGTATTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT160475 WP_003086329.1 NZ_CP054793:c4390647-4390279 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT160475 NZ_CP070521:8754-8990 [Morganella morganii]
ATGGCTGTAACACAACGACCAATGCAAACCGTAACCATGACGGTAAAACGAACCTTGCTCACCCGTGCGCGTGAGGCTGG
AATTAATTTGAGCGCCACCCTGACCGCTGCCCTCGATGCTGAATTACGTCAGTACGAAGCCAAGAGGTGGCAAGAGGAAA
ACAGCGAAGCACTGGAAGCCCTCAATCGCTTCCACGACGAACACGGCTGTTTCAGTGATGAATACAGGACATTTTAA

Structures

Antitoxin

• AlphaFold DB: V6AJU7