Detailed information of TA system TA160474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3468887..3470108
• Replicon: chromosome
• Accession: NZ_CP054793
• Organism: Pseudomonas aeruginosa strain SE5452

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: HUK77_RS15830
• Protein ID: WP_003102740.1
• Coordinates: 3469530..3470108 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUK77_RS15825
• Protein ID: WP_003088112.1
• Coordinates: 3468887..3469441 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK77_RS15800 (HUK77_15810)	3464455..3465006	+	552	WP_003132721.1	XRE family transcriptional regulator	-
HUK77_RS15805 (HUK77_15815)	3465003..3465401	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HUK77_RS15810 (HUK77_15820)	3465414..3465737	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
HUK77_RS15815 (HUK77_15825)	3466068..3466529	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HUK77_RS15820 (HUK77_15830)	3466534..3468729	+	2196	WP_014603034.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HUK77_RS15825 (HUK77_15835)	3468887..3469441	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
HUK77_RS15830 (HUK77_15840)	3469530..3470108	+	579	WP_003102740.1	HD domain-containing protein	Toxin
HUK77_RS15835 (HUK77_15845)	3470216..3471403	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HUK77_RS15840 (HUK77_15850)	3471393..3473564	-	2172	WP_014603035.1	type I secretion system permease/ATPase	-
HUK77_RS15845 (HUK77_15855)	3473554..3474831	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T160474 WP_003102740.1 NZ_CP054793:3469530-3470108 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T160474 NZ_CP070521:2219-2635 [Morganella morganii]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT160474 WP_003088112.1 NZ_CP054793:3468887-3469441 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT160474 NZ_CP070521:1992-2222 [Morganella morganii]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07