Detailed information of TA system TA160473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1820741..1822249
• Replicon: chromosome
• Accession: NZ_CP054793
• Organism: Pseudomonas aeruginosa strain SE5452

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HUK77_RS08465
• Protein ID: WP_012613851.1
• Coordinates: 1820741..1821328 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HUK77_RS08470
• Protein ID: WP_235195500.1
• Coordinates: 1821395..1822249 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK77_RS08460 (HUK77_08470)	1816996..1820475	-	3480	WP_003117605.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
HUK77_RS08465 (HUK77_08475)	1820741..1821328	+	588	WP_012613851.1	GNAT family protein	Toxin
HUK77_RS08470 (HUK77_08480)	1821395..1822249	+	855	WP_235195500.1	AraC family transcriptional regulator	Antitoxin
HUK77_RS08475 (HUK77_08485)	1822346..1824511	+	2166	WP_003115567.1	TonB-dependent receptor family protein	-
HUK77_RS08480 (HUK77_08490)	1824686..1826560	+	1875	WP_014602678.1	MFS transporter	-
HUK77_RS08485 (HUK77_08495)	1826621..1826830	-	210	WP_003115568.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21929.29 Da
• Isoelectric Point: 9.8676

>T160473 WP_012613851.1 NZ_CP054793:1820741-1821328 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAVLEASFTG

Nucleotide

• Length: 588 bp

>T160473 NZ_CP070520:c4749917-4749699 [Klebsiella aerogenes]
ATGTCTGGTAAACCATTAACTAAAACAGACTATTTGATGCGTTTACGCCGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAGAATAAATATGAACTTTCCGACAATGAATTGGCGGTATTTTACTCAGCAGCCGACCATCGTCTTGCTGAAT
TGACCATGAATAAGCTATACGACAAAATCCCAACTTCAGTATGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31919.20 Da
• Isoelectric Point: 7.3276

>AT160473 WP_235195500.1 NZ_CP054793:1821395-1822249 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHVAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 855 bp

>AT160473 NZ_CP070520:c4750318-4749944 [Klebsiella aerogenes]
ATGGACGAGTACTCGCCAAAAAGACATGATATCGCGCAGCTAAGATTTCTCTGCGAAACGTTGTATCATGACTGCCTTGC
AAACCTGGAAGAAAGCAACCGCGGCTGGGTTAACGATCCCACCTCGGCGGTGAATCTTCAATTAAATGAATTGATCGAGC
ACATAGCAACTTACGCGCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATTTCTCAGCTTGACGAATACCTG
GATGACACTTTTATGCTGTTCAGTAGCTATGGCATAAACACAAGCGATCTCCAAAAATGGCGCAAATCCGGCAATCGGTT
ATTCCGATGCTTTGTGAACGCCAGCCGGGAGAACCCGGCAAGTCTTTCTTGTTAG