Detailed information of TA system TA160470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5311355..5311995
• Replicon: chromosome
• Accession: NZ_CP054792
• Organism: Pseudomonas aeruginosa strain SE5431

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUK75_RS25030
• Protein ID: WP_003105740.1
• Coordinates: 5311355..5311765 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUK75_RS25035
• Protein ID: WP_031634724.1
• Coordinates: 5311765..5311995 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK75_RS24990	5306707..5307420	+	714	WP_235179931.1	hypothetical protein	-
HUK75_RS24995 (HUK75_24945)	5307577..5308305	+	729	WP_025324783.1	TIGR03761 family integrating conjugative element protein	-
HUK75_RS25000 (HUK75_24950)	5308311..5308859	+	549	WP_034038747.1	DUF3158 family protein	-
HUK75_RS25005 (HUK75_24955)	5308907..5309737	+	831	WP_023132184.1	Rha family transcriptional regulator	-
HUK75_RS25010 (HUK75_24960)	5309767..5310255	+	489	WP_034038749.1	single-stranded DNA-binding protein	-
HUK75_RS25015 (HUK75_24965)	5310393..5310590	+	198	WP_003109353.1	CrpP family ICE-associated protein	-
HUK75_RS25020 (HUK75_24970)	5310656..5310874	-	219	WP_003105747.1	hypothetical protein	-
HUK75_RS25025 (HUK75_24975)	5311073..5311333	-	261	WP_003105742.1	hypothetical protein	-
HUK75_RS25030 (HUK75_24980)	5311355..5311765	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HUK75_RS25035 (HUK75_24985)	5311765..5311995	-	231	WP_031634724.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HUK75_RS25040 (HUK75_24990)	5312313..5314232	+	1920	WP_034038751.1	type I DNA topoisomerase	-
HUK75_RS25045 (HUK75_24995)	5314241..5314993	+	753	WP_034038753.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5292188..5391306	99118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T160470 WP_003105740.1 NZ_CP054792:c5311765-5311355 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T160470 NZ_CP070520:c1618433-1618330 [Klebsiella aerogenes]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT160470 WP_031634724.1 NZ_CP054792:c5311995-5311765 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT160470 NZ_CP070520:1618322-1618468 [Klebsiella aerogenes]
TAATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAA
GTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTGACTGTGACAGGTTTTCCAGT