Detailed information of TA system TA160469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4469417..4470675
• Replicon: chromosome
• Accession: NZ_CP054792
• Organism: Pseudomonas aeruginosa strain SE5431

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: HUK75_RS20760
• Protein ID: WP_003086331.1
• Coordinates: 4469417..4470172 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HUK75_RS20765
• Protein ID: WP_003086329.1
• Coordinates: 4470307..4470675 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK75_RS20750 (HUK75_20720)	4464585..4467128	+	2544	WP_019486194.1	acyl-homoserine-lactone acylase QuiP	-
HUK75_RS20755 (HUK75_20725)	4467283..4468644	-	1362	WP_003134034.1	DNA recombination protein RmuC	-
HUK75_RS20760 (HUK75_20730)	4469417..4470172	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
HUK75_RS20765 (HUK75_20735)	4470307..4470675	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HUK75_RS20770 (HUK75_20740)	4470748..4472034	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
HUK75_RS20775 (HUK75_20745)	4472090..4473679	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
HUK75_RS20780 (HUK75_20750)	4473757..4474233	+	477	WP_003086322.1	hypothetical protein	-
HUK75_RS20785 (HUK75_20755)	4474236..4474511	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T160469 WP_003086331.1 NZ_CP054792:c4470172-4469417 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T160469 NZ_CP070520:1257427-1257723 [Klebsiella aerogenes]
ATGCAGAATCACATAGGTAGCTTTCGTGATGTATGGTTGGAAGAGTTTTTTTTGCACTCGGCACCACATAAAAAAATTCC
TCCAGACATACATACCACGCTAGCTCGCAAACTCGATATCATCAATGCCGCCGTTACCTATCTGGATTTACGCTCGCCAC
CTGGTAATAGATATGAAGAGCTAAACGGCAAATTGGAAGGTTATTCGTCAATTAGAATTAACCAGCAGTACCGCTTAATA
TTTAAGTGGGTGGATGGAAAAGCGGAAGACCTTTACCTGGATCCGCACAAGTACTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT160469 WP_003086329.1 NZ_CP054792:c4470675-4470307 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT160469 NZ_CP070520:1257781-1258116 [Klebsiella aerogenes]
ATGAAACAGGCAACCAGAAAACCAACTACCGTAGGTGACATCCTGCAGTTTGAATACCTGGAGCCGCTAGATTTAAAAAT
CAACGAACTTGCGGAAATGATACATGTGCATCGTAATAGTGTAAGTGCATTAGTTAACAACAACCGCAAACTAACTACTG
ACATGGCATTTCGCCTGTCTAAAGCCTTCAATACCACCGTTGATTTCTGGCTTAACCTTCAGGCTGCTGTAGATTTGTGG
GAGGTGGAGAATGACACCCGCGCGCAGGAAGAGTTTAGCCGCATTGTTCCCGCCTCGAAATTTATCGCCAGCAAAGCTGA
TAAGAAAGTCGCCTGA

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7