Detailed information of TA system TA160466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 144807..145312
• Replicon: chromosome
• Accession: NZ_CP054792
• Organism: Pseudomonas aeruginosa strain SE5431

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: HUK75_RS00670
• Protein ID: WP_003083773.1
• Coordinates: 144807..145088 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: HUK75_RS00675
• Protein ID: WP_003083775.1
• Coordinates: 145085..145312 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK75_RS00645 (HUK75_00645)	140058..141407	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
HUK75_RS00650 (HUK75_00650)	141456..142142	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
HUK75_RS00655 (HUK75_00655)	142243..142977	+	735	WP_003110658.1	GntR family transcriptional regulator	-
HUK75_RS00660 (HUK75_00660)	143157..143567	+	411	WP_003110659.1	aegerolysin family protein	-
HUK75_RS00665 (HUK75_00665)	143599..144507	-	909	WP_003083769.1	LysR family transcriptional regulator	-
HUK75_RS00670 (HUK75_00670)	144807..145088	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
HUK75_RS00675 (HUK75_00675)	145085..145312	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
HUK75_RS00680 (HUK75_00680)	145488..146108	-	621	WP_003101226.1	hypothetical protein	-
HUK75_RS00685 (HUK75_00685)	146209..146709	+	501	WP_033990064.1	LEA type 2 family protein	-
HUK75_RS00690 (HUK75_00690)	146782..147123	+	342	WP_024947024.1	zinc ribbon domain-containing protein YjdM	-
HUK75_RS00695 (HUK75_00695)	147205..148632	-	1428	WP_023092260.1	GABA permease	-
HUK75_RS00700 (HUK75_00700)	148800..150293	-	1494	WP_024947023.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T160466 WP_003083773.1 NZ_CP054792:c145088-144807 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T160466 NZ_CP070511:c262150-261800 [Parageobacillus toebii]
TTGATTGTTAAGCGTGGCGACGTTTATTTTGCGGACCTTTCCCCGGTAGTGGGCTCAGAGCAAGGAGGCGTTCGTCCGGT
ATTGGTCATTCAAAATGACATTGGAAATCGATTTAGTCCGACGGTGATTGTGGCGGCAATTACAGCACAAATTCAAAAAG
CGAAGCTGCCAACGCATGTCGAAATTGATGCAAAACGTTACGGGTTTGAGCGGGATTCTGTCATTCTGCTAGAGCAAATT
CGGACAATTGATAAACAGCGGCTAACAGACAAAATTACTCACTTGGACGATGAAATGATGGATAAGGTGGACGAAGCGCT
GCAAATTAGTTTAGGACTTATAGATTTTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT160466 WP_003083775.1 NZ_CP054792:c145312-145085 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT160466 NZ_CP070511:c262436-262155 [Parageobacillus toebii]
GTGTCGGAATCTAGCGCAACAACAGAAATCGTCGTTCGTTTGCCTCAAGCATTGGTAACCGAATTAGATGTGCTTGTAAA
ACAGGAAAACGGCAACCGCAATGACCTTATTTATCAAGCGACTAAAATGTATATTCGCGAGCGAAAAAAACGTCAAATCC
GTGAAGCAATGAGACGCGGTTATATGGAAATGGCCAAAATCAATTTATCCATTGCTTCAGAAGCGTTTCTTGCAGAATAC
GAGGCCGACCACACCGTTGAACGTTTAGTTAGCGGGGGGTAA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9