Detailed information of TA system TA160462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3279508..3280729
• Replicon: chromosome
• Accession: NZ_CP054791
• Organism: Pseudomonas aeruginosa strain SE5430

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: V6AG16
• Locus tag: HUK74_RS15605
• Protein ID: WP_003088107.1
• Coordinates: 3279508..3280086 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUK74_RS15610
• Protein ID: WP_003088112.1
• Coordinates: 3280175..3280729 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK74_RS15590 (HUK74_15555)	3274784..3276061	+	1278	WP_003088104.1	TolC family protein	-
HUK74_RS15595 (HUK74_15560)	3276051..3278222	+	2172	WP_003088105.1	type I secretion system permease/ATPase	-
HUK74_RS15600 (HUK74_15565)	3278212..3279399	+	1188	WP_003088106.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HUK74_RS15605 (HUK74_15570)	3279508..3280086	-	579	WP_003088107.1	HD domain-containing protein	Toxin
HUK74_RS15610 (HUK74_15575)	3280175..3280729	-	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
HUK74_RS15615 (HUK74_15580)	3280887..3283082	-	2196	WP_003088114.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HUK74_RS15620 (HUK74_15585)	3283087..3283548	-	462	WP_003088117.1	(2Fe-2S)-binding protein	-
HUK74_RS15625 (HUK74_15590)	3283879..3284202	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
HUK74_RS15630 (HUK74_15595)	3284215..3284613	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HUK74_RS15635 (HUK74_15600)	3284610..3285161	-	552	WP_023098678.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-187)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21885.74 Da
• Isoelectric Point: 5.3655

>T160462 WP_003088107.1 NZ_CP054791:c3280086-3279508 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDAKTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T160462 NZ_CP070508:4160584-4160928 [Serratia ureilytica]
ATGTGGCAAGTGGTGACCGTCGAACGATTCGACGACTGGTTTTTGGCGCTGAACAACGCCCAGCAAACAAGCATCCTGGC
GGCGATCTTCAAATTACAGACGTTCGGCCCGCAGTTGGCGCGGCCGCACGCCGATACGCTGCATTTTTCCGAAGCGGCCC
GGCAGTTGAAGGAGTTGCGCGTTCAACATCGCGGGCGCCCTTTCAGGGTGTTTTTCGCCTTTGACCCACAGCGGCAAGCA
GTATTGCTGTGCGGTGGCGATAAAACCGGCGACAAACGCTTTTACCAGCGCATGTTGCCCATCGCTGCCATGGAATTTTC
ACATTATCTGGCAACCCGGAGGTAG

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT160462 WP_003088112.1 NZ_CP054791:c3280729-3280175 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT160462 NZ_CP070508:4160934-4161236 [Serratia ureilytica]
ATGGCCAAACCGTTATCCCAACTGATCGACAAGCTCGATCCCGCCGTCGTTGCCGCAGCCAGGCAAAAAGCGGACAAGGA
AATTTTCGAACTGCGGCTGGCAATGCTGAGGGAAGAGCTGGCGGTCTCTCAGGTTGAATTGGCGAAGCGGCTCGGTATCA
GCCAACCCTCCGTCGCCAATTTGGAAAAGCGCGGCAGCGAAATAAAGCTGTCGTCGCTCAAACGCTATATCGAGGCGATG
GGCGGCACGCTGTCGCTGGATGTGCAATTGCCCAATGGCCAGCACCGTCGCATGACGCTGTGA

Structures

Toxin

• AlphaFold DB: V6AG16