Detailed information of TA system TA160461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1926165..1927673
• Replicon: chromosome
• Accession: NZ_CP054791
• Organism: Pseudomonas aeruginosa strain SE5430

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HUK74_RS08985
• Protein ID: WP_073660237.1
• Coordinates: 1926165..1926752 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A1C7C4U7
• Locus tag: HUK74_RS08990
• Protein ID: WP_003091607.1
• Coordinates: 1926819..1927673 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK74_RS08980 (HUK74_08970)	1922420..1925899	-	3480	WP_003091610.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
HUK74_RS08985 (HUK74_08975)	1926165..1926752	+	588	WP_073660237.1	GNAT family protein	Toxin
HUK74_RS08990 (HUK74_08980)	1926819..1927673	+	855	WP_003091607.1	AraC family transcriptional regulator	Antitoxin
HUK74_RS08995 (HUK74_08985)	1927770..1929935	+	2166	WP_003091606.1	TonB-dependent receptor family protein	-
HUK74_RS09000 (HUK74_08990)	1930110..1931984	+	1875	WP_003091605.1	MFS transporter	-
HUK74_RS09005 (HUK74_08995)	1932045..1932254	-	210	WP_003091604.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21929.29 Da
• Isoelectric Point: 9.8676

>T160461 WP_073660237.1 NZ_CP054791:1926165-1926752 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLVEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T160461 NZ_CP070508:2036161-2036257 [Serratia ureilytica]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31895.20 Da
• Isoelectric Point: 7.5295

>AT160461 WP_003091607.1 NZ_CP054791:1926819-1927673 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETMLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Nucleotide

• Length: 855 bp

>AT160461 NZ_CP070508:c2036257-2036117 [Serratia ureilytica]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG

Structures

Antitoxin

• AlphaFold DB: A0A1C7C4U7