Detailed information of TA system TA160460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 147641..148146
• Replicon: chromosome
• Accession: NZ_CP054791
• Organism: Pseudomonas aeruginosa strain SE5430

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: HUK74_RS00660
• Protein ID: WP_003083773.1
• Coordinates: 147641..147922 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: HUK74_RS00665
• Protein ID: WP_003083775.1
• Coordinates: 147919..148146 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK74_RS00635 (HUK74_00630)	142892..144241	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
HUK74_RS00640 (HUK74_00635)	144290..144976	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
HUK74_RS00645 (HUK74_00640)	145077..145811	+	735	WP_003083764.1	GntR family transcriptional regulator	-
HUK74_RS00650 (HUK74_00645)	145991..146401	+	411	WP_003110659.1	aegerolysin family protein	-
HUK74_RS00655 (HUK74_00650)	146433..147341	-	909	WP_003083769.1	LysR family transcriptional regulator	-
HUK74_RS00660 (HUK74_00655)	147641..147922	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
HUK74_RS00665 (HUK74_00660)	147919..148146	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
HUK74_RS00670 (HUK74_00665)	148322..148942	-	621	WP_003101226.1	hypothetical protein	-
HUK74_RS00675 (HUK74_00670)	149043..149543	+	501	WP_003083778.1	LEA type 2 family protein	-
HUK74_RS00680 (HUK74_00675)	149616..149957	+	342	WP_014603467.1	zinc ribbon domain-containing protein YjdM	-
HUK74_RS00685 (HUK74_00680)	150039..151466	-	1428	WP_003083784.1	GABA permease	-
HUK74_RS00690 (HUK74_00685)	151635..153128	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T160460 WP_003083773.1 NZ_CP054791:c147922-147641 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T160460 NZ_CP070508:c1768157-1767873 [Serratia ureilytica]
ATGACTTATAAGCTTGAATTCGAAGAACATGCGCTGAAGGAATTTAAAAAACTGTCCCCGGTGATCCGGGAACAGTTCAA
AAAAAAGCTGGCATCGGTGCTGGTTAATCCCCACGTCCCCGCCAACAAACTCGCCGGTTTGCCCGATTGCTACAAAATCA
AACTCAGAGCCTCAGGCTTTCGTTTGGTATATCGCGTCATGGAAACGGAGATCGTGGTTCTGGTCCTGTCTGTGGGCAAA
CGCGAACGTTCAGCGGCTTACACGGCTGCCAAAAAGCGCCTTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT160460 WP_003083775.1 NZ_CP054791:c148146-147919 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT160460 NZ_CP070508:c1768398-1768147 [Serratia ureilytica]
ATGACGGTTCAACCCATTCTTGCCGACTCGGCTGTCAGCATCAGCGATTTTAAGAAATCGCCCAACGCCGCCCTTAAAGA
GGCACAAGGGCAACCGGTCGCCGTCCTGACCAACGGTAAGATTTCGGGATATTACGTCTCTCCGGAAACCTGGGAGGCGA
TCGCGGAATACCTGGAAGATATCGAACTCGCTGAAATCGTTCGTTCACGGATGAACGGCAAACGCATTAAGGTCAATTTG
GATGACTTATAA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9