Detailed information of TA system TA160458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5604071..5604657
• Replicon: chromosome
• Accession: NZ_CP054790
• Organism: Pseudomonas aeruginosa strain SE5418

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HUK71_RS26710
• Protein ID: WP_003120987.1
• Coordinates: 5604358..5604657 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HUK71_RS26705
• Protein ID: WP_003448662.1
• Coordinates: 5604071..5604361 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK71_RS26690 (HUK71_26685)	5599665..5601542	+	1878	WP_023911870.1	hypothetical protein	-
HUK71_RS26695 (HUK71_26690)	5601539..5603515	+	1977	WP_031275715.1	DEAD/DEAH box helicase	-
HUK71_RS32675	5603525..5603659	+	135	WP_033179080.1	hypothetical protein	-
HUK71_RS26700 (HUK71_26695)	5603656..5604000	+	345	WP_003448665.1	hypothetical protein	-
HUK71_RS26705 (HUK71_26700)	5604071..5604361	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HUK71_RS26710 (HUK71_26705)	5604358..5604657	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK71_RS26715 (HUK71_26710)	5604858..5605982	+	1125	WP_235176245.1	TcpQ domain-containing protein	-
HUK71_RS26720 (HUK71_26715)	5605986..5607692	+	1707	WP_061195893.1	PilN family type IVB pilus formation outer membrane protein	-
HUK71_RS26725 (HUK71_26720)	5607696..5609021	+	1326	WP_023435878.1	type 4b pilus protein PilO2	-
HUK71_RS26730 (HUK71_26725)	5609011..5609544	+	534	WP_023435877.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T160458 WP_003120987.1 NZ_CP054790:c5604657-5604358 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T160458 NZ_CP070508:209585-209860 [Serratia ureilytica]
ATGCTCAACATCGAATGGACTCGAGCCGCACGTCAGGATCTGGCGGAGATTGTGGGATTCATCGCCAACGATAACCCTCT
GGCGGCCAGAAAAATGAAGGTTCTCATCATGGAGGCCGTCACTCCCGCCGCCAGGCATCCTCGAATTTTCCGGCGTGGCA
GAGTGGACGGCACGCATGAAATCCCCTTGCATCCCCATTATCTCCTGATTTACCAGGTGCTGGAAACTCACATCCGGGTG
TTGCGGGTGTTACATACGCGCCGACAATATCCTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT160458 WP_003448662.1 NZ_CP054790:c5604361-5604071 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT160458 NZ_CP070508:209385-209585 [Serratia ureilytica]
ATGATGAGTATTTTACTACTGCCTACGGTTGCCAAGATGGAAAATGAGGAACACACACAGGGCTATGAGCGCTGGTTTCT
TGAAAGAGTTCAGGAAGCCATGGATGAGCCGTGGCCCGGTATTCCTCATGATGACGTGATGGCTGAAATGGATGCTGTTA
TCGCTGCTGCAGAAGCCAGGATCAACGCGAAGCAGGCATAA

Structures

Toxin

• AlphaFold DB: G8CP73