Detailed information of TA system TA160457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4825969..4826609
• Replicon: chromosome
• Accession: NZ_CP054790
• Organism: Pseudomonas aeruginosa strain SE5418

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUK71_RS22925
• Protein ID: WP_003105740.1
• Coordinates: 4825969..4826379 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUK71_RS22930
• Protein ID: WP_031634724.1
• Coordinates: 4826379..4826609 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK71_RS22885	4821321..4822034	+	714	WP_235179931.1	hypothetical protein	-
HUK71_RS22890 (HUK71_22885)	4822191..4822919	+	729	WP_025324783.1	TIGR03761 family integrating conjugative element protein	-
HUK71_RS22895 (HUK71_22890)	4822925..4823473	+	549	WP_034038747.1	DUF3158 family protein	-
HUK71_RS22900 (HUK71_22895)	4823521..4824351	+	831	WP_023132184.1	Rha family transcriptional regulator	-
HUK71_RS22905 (HUK71_22900)	4824381..4824869	+	489	WP_034038749.1	single-stranded DNA-binding protein	-
HUK71_RS22910 (HUK71_22905)	4825007..4825204	+	198	WP_003109353.1	CrpP family ICE-associated protein	-
HUK71_RS22915 (HUK71_22910)	4825270..4825488	-	219	WP_003105747.1	hypothetical protein	-
HUK71_RS22920 (HUK71_22915)	4825687..4825947	-	261	WP_003105742.1	hypothetical protein	-
HUK71_RS22925 (HUK71_22920)	4825969..4826379	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HUK71_RS22930 (HUK71_22925)	4826379..4826609	-	231	WP_031634724.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HUK71_RS22935 (HUK71_22930)	4826927..4828846	+	1920	WP_034038751.1	type I DNA topoisomerase	-
HUK71_RS22940 (HUK71_22935)	4828855..4829607	+	753	WP_034038753.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4797903..4833816	35913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T160457 WP_003105740.1 NZ_CP054790:c4826379-4825969 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T160457 NZ_CP070508:62441-62716 [Serratia ureilytica]
ATGGAGTTAAAATGGACGAGTAAGGCGCTACCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTCGATAATCCTCGCCTGGGTGAAAGACTGGACG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGATGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT160457 WP_031634724.1 NZ_CP054790:c4826609-4826379 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT160457 NZ_CP070508:62181-62441 [Serratia ureilytica]
ATGAATACGAAAGTCATCACCGCTCATGTCCCGCTGCCTTTGGCCGAAAAGGTGGATGAAATGGCCGCCAAACTTGAACG
CTCACGGGGATGGATAGTGAAGCAAGCCCTCGCCGCCTGGCTTGAGCAAGAGGAAGAGCGCCGTCGGTTAACGCTGGCGG
CGCTGGCCGATGTCGAACAGCAAAACGTTATCGATCATGAGTCGGTAGAAGCCTGGGCGGCCAGCCTGGACAGTGACGAG
CCGCTGCCGGTACCGCGCTGA