Detailed information of TA system TA160456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4749860..4751118
• Replicon: chromosome
• Accession: NZ_CP054790
• Organism: Pseudomonas aeruginosa strain SE5418

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: HUK71_RS22505
• Protein ID: WP_003112528.1
• Coordinates: 4749860..4750615 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HUK71_RS22510
• Protein ID: WP_003086329.1
• Coordinates: 4750750..4751118 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK71_RS22495 (HUK71_22495)	4745033..4747576	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
HUK71_RS22500 (HUK71_22500)	4747731..4749092	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
HUK71_RS22505 (HUK71_22505)	4749860..4750615	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
HUK71_RS22510 (HUK71_22510)	4750750..4751118	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HUK71_RS22515 (HUK71_22515)	4751191..4752477	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
HUK71_RS22520 (HUK71_22520)	4752533..4754122	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
HUK71_RS22525 (HUK71_22525)	4754201..4754677	+	477	WP_003112531.1	hypothetical protein	-
HUK71_RS22530 (HUK71_22530)	4754680..4754955	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T160456 WP_003112528.1 NZ_CP054790:c4750615-4749860 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T160456 NZ_CP070508:c14523-14143 [Serratia ureilytica]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAGGGACGTTCT
GGTCGAGTATCACATCAATCCGGCCATCAGCGCCATCACCTGGATGGAAGTGATGGTAGGTGCTAAAAAACAGGGTCCGG
CACTGGAGCTGAAAACACGTCAGTTTTTAGGGCAATTTCTGCTGCTGCCTATTACCGATGAAGTCGCCGAACGCGCCGTA
GAACTGCGCCACTCACAGCATGTGAAACTGCCAGACGCCATTATCTGGGCCACAGCGCAGGTGGGATTCCGGATGCTCAT
CTCCCGCAACCCCAAAGATTTCGGCACTGACAATGGCGTGTTGATGCCTTATCGGCTGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT160456 WP_003086329.1 NZ_CP054790:c4751118-4750750 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT160456 NZ_CP070508:c14744-14523 [Serratia ureilytica]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGATAATCTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATTCGTCAAGCAGTCTCCCGCTATCTGACGGAGAACCACGTGGAAGATCCCAGCAACGCTTTCGGGCTCT
GGTCCGGGGCGATGGGAGATGGCGTGGAGTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7