Detailed information of TA system TA160450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5444009..5444595
• Replicon: chromosome
• Accession: NZ_CP054789
• Organism: Pseudomonas aeruginosa strain SE5381

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HUK70_RS25860
• Protein ID: WP_003120987.1
• Coordinates: 5444296..5444595 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HUK70_RS25855
• Protein ID: WP_003448662.1
• Coordinates: 5444009..5444299 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK70_RS25840 (HUK70_25760)	5439603..5441480	+	1878	WP_023911870.1	hypothetical protein	-
HUK70_RS25845 (HUK70_25765)	5441477..5443453	+	1977	WP_031275715.1	DEAD/DEAH box helicase	-
HUK70_RS32080	5443463..5443597	+	135	WP_033179080.1	hypothetical protein	-
HUK70_RS25850 (HUK70_25770)	5443594..5443938	+	345	WP_003448665.1	hypothetical protein	-
HUK70_RS25855 (HUK70_25775)	5444009..5444299	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HUK70_RS25860 (HUK70_25780)	5444296..5444595	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK70_RS25865 (HUK70_25785)	5444796..5445920	+	1125	WP_235176245.1	TcpQ domain-containing protein	-
HUK70_RS25870 (HUK70_25790)	5445920..5447629	+	1710	WP_003123331.1	PilN family type IVB pilus formation outer membrane protein	-
HUK70_RS25875 (HUK70_25795)	5447633..5448958	+	1326	WP_023435878.1	type 4b pilus protein PilO2	-
HUK70_RS25880 (HUK70_25800)	5448948..5449481	+	534	WP_023435877.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T160450 WP_003120987.1 NZ_CP054789:c5444595-5444296 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T160450 NZ_CP070506:3594351-3594695 [Pseudomonas sp. SDM007]
ATGGATGCTCTATTTATCGAACTGCCGCCCTTTGAGCGGTACCGCAAGGACTACCTGACTGACGAACTGTTTCACGGCTT
TCAACAAGAGCTAATGAAGAACCCCGAAGCGGGAGCCGTGATGGAAGGCACCGGTGGGTTACGCAAGCTGCGCTTCGTCG
ATGAACGACGAAACAAAGGCAAACGGGGTGGATTACGCGTCATCTACTACTGGTGGTCAGGCGGCACTCAATTTTGGTTG
TTCACGCTGTACGGCAAGCACGAGCAAAGCGACTTGAGCCCACAACACAAAAAAGCCCTCAAGCAACTGCTGGACAGGGA
AATCAACGCGAGAGGATTCACATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT160450 WP_003448662.1 NZ_CP054789:c5444299-5444009 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT160450 NZ_CP070506:3594692-3595006 [Pseudomonas sp. SDM007]
ATGAAACGTGACATTTATTCCGAACTGGTCGAAGGCTTCGATGCCTTGGCCAACGAGCGCCAAGGCAAGGTCACCCTACG
TACTCATACCGTGCAGCAAAACAAACTGGCCCCTCTTACCGCTGAAGACGTGATTGCAGTACGACAACAACTCAATCTTT
CCCGTGCTGTATTTGCCATGTACCTACGCACCAATACACGCACCCTGGAAAACTGGGAGCAAGGTCGCGCACGCCCCAAT
GCCCAGGCAGCCACGCTGATTCGTCTGGTATCAAAATTTCCGGATACGGTGGAGCGCCTGGCGTTGCTTAGCTGA

Structures

Toxin

• AlphaFold DB: G8CP73