Detailed information of TA system TA160449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4743979..4745237
• Replicon: chromosome
• Accession: NZ_CP054789
• Organism: Pseudomonas aeruginosa strain SE5381

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: HUK70_RS22535
• Protein ID: WP_003112528.1
• Coordinates: 4743979..4744734 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HUK70_RS22540
• Protein ID: WP_003086329.1
• Coordinates: 4744869..4745237 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK70_RS22525 (HUK70_22450)	4739152..4741695	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
HUK70_RS22530 (HUK70_22455)	4741850..4743211	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
HUK70_RS22535 (HUK70_22460)	4743979..4744734	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
HUK70_RS22540 (HUK70_22465)	4744869..4745237	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HUK70_RS22545 (HUK70_22470)	4745310..4746596	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
HUK70_RS22550 (HUK70_22475)	4746652..4748241	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
HUK70_RS22555 (HUK70_22480)	4748320..4748796	+	477	WP_003112531.1	hypothetical protein	-
HUK70_RS22560 (HUK70_22485)	4748799..4749074	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T160449 WP_003112528.1 NZ_CP054789:c4744734-4743979 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T160449 NZ_CP070506:c3027864-3027589 [Pseudomonas sp. SDM007]
ATGGAGTTGAAGTGGACGAGCAGCGCGCTGTCCGACATTAGCCGGCTTTATGAGTTTCTGGCCGCGGTGAATCAATCTGC
CGCCGCACACGCCGTGCAGCGCCTCACAGCCGCGCCAACGACGCTGTTGGCCAACCCACGCATCGGCGAACGTCTGGAAG
AGTTCGAAGCAAGGGATGTGCGCCGAATTCAGATCGGCCAGTATGAGATGCGCTACGAAATAGTGGACTCCACAATCTAT
CTACTGCGCCTGTGGCACACCCGGGAGGATCGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT160449 WP_003086329.1 NZ_CP054789:c4745237-4744869 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT160449 NZ_CP070506:c3028136-3027864 [Pseudomonas sp. SDM007]
ATGGCAACCCCAGGGAAAACCCGATCTGTCACGGCTCACGTCCCTGAGCAACTGGCCCAAAAAGTCGATTTGATGGCAGA
ACGCCTGGAGCGCTCCAAAAACTGGATCGTCAGACAGGCGCTCTCCGCTTGGATCGACCAGGAAGAAGAACGTAGCCGAC
TGACACGCGAAGCCTTGGCAGATGTTGACGCTGGACGCGTCATTGATCACCAAGCAGTCCAAGCCTGGGCGGACAGCCTA
AGCACCGACGCGCCATTGCCGGTACCACGCTAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7