Detailed information of TA system TA160448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3764811..3766032
• Replicon: chromosome
• Accession: NZ_CP054789
• Organism: Pseudomonas aeruginosa strain SE5381

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: HUK70_RS17895
• Protein ID: WP_003102740.1
• Coordinates: 3765454..3766032 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUK70_RS17890
• Protein ID: WP_003088112.1
• Coordinates: 3764811..3765365 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK70_RS17865 (HUK70_17800)	3760379..3760930	+	552	WP_004343115.1	XRE family transcriptional regulator	-
HUK70_RS17870 (HUK70_17805)	3760927..3761325	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HUK70_RS17875 (HUK70_17810)	3761338..3761661	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
HUK70_RS17880 (HUK70_17815)	3761992..3762453	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HUK70_RS17885 (HUK70_17820)	3762458..3764653	+	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HUK70_RS17890 (HUK70_17825)	3764811..3765365	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
HUK70_RS17895 (HUK70_17830)	3765454..3766032	+	579	WP_003102740.1	HD domain-containing protein	Toxin
HUK70_RS17900 (HUK70_17835)	3766141..3767328	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HUK70_RS17905 (HUK70_17840)	3767318..3769489	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
HUK70_RS17910 (HUK70_17845)	3769479..3770756	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T160448 WP_003102740.1 NZ_CP054789:3765454-3766032 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T160448 NZ_CP070506:c2217218-2216967 [Pseudomonas sp. SDM007]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAACCTGCAGATGGCATTTACCTGGCCTGAGCTCATCACGTTGCT
GGGCTGGCTGGGTTATCGACAAATCGAAGGGGCTGGGAGCCGCGTCAAGTTCGACATCGGCGACCCGAGTGCAATGATCA
CCCTGCACAAGCCCCACCCTGGCAATGAGCTGAAACACTACATTCGGCGCCAGATCATTGAACAGTTGGAATCAGGAGGC
TTGCTTCAATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT160448 WP_003088112.1 NZ_CP054789:3764811-3765365 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT160448 NZ_CP070506:c2216970-2216605 [Pseudomonas sp. SDM007]
ATGAGCAACCAGTTGAAACACAAAGGCTACATCGGCTCCATAGAAGCCAGCGTCGAGGACAATTGCCTGTTCGGCAAGCT
GCTGTTTATCCGCGCCCTGGTCAGCTACGAAGGCCAGACCGTGGCCCAGTTGGAAGCGGCCTTTCGCGAAGCCGTGGAGG
ATTACCTGGCTACATGCCAGACACTCGGCCAGGCACCGGAAAAGCCCTGCAAAGGCTCATTCAATGTGCGGGTCGGCCAT
GATCTGCACCTGGCCGCGGCGCTGGCGGCGACGCAGAAAAAGGTCACGCTCAACGACCTGACGCGCCAGGCGTTGAACGA
GTTCCTGCGTCATCACGCTACGGATATTCACCCCGCCCTGGGGTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07