Detailed information of TA system TA160443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5467158..5467744
• Replicon: chromosome
• Accession: NZ_CP054788
• Organism: Pseudomonas aeruginosa strain YTSY4

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HUK69_RS25995
• Protein ID: WP_003120987.1
• Coordinates: 5467445..5467744 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HUK69_RS25990
• Protein ID: WP_003448662.1
• Coordinates: 5467158..5467448 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK69_RS25970 (HUK69_25940)	5462309..5462518	+	210	WP_003105733.1	cold-shock protein	-
HUK69_RS25975 (HUK69_25945)	5462740..5464629	+	1890	WP_016851610.1	hypothetical protein	-
HUK69_RS25980 (HUK69_25950)	5464626..5466602	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
HUK69_RS25985 (HUK69_25955)	5466743..5467087	+	345	WP_016851612.1	hypothetical protein	-
HUK69_RS25990 (HUK69_25960)	5467158..5467448	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HUK69_RS25995 (HUK69_25965)	5467445..5467744	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK69_RS26000 (HUK69_25970)	5467946..5469070	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
HUK69_RS26005 (HUK69_25975)	5469070..5470779	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
HUK69_RS26010 (HUK69_25980)	5470783..5472108	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
HUK69_RS26015 (HUK69_25985)	5472098..5472631	+	534	WP_003120993.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5439373..5529070	89697

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T160443 WP_003120987.1 NZ_CP054788:c5467744-5467445 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T160443 NZ_CP070503:5717179-5717577 [Pseudomonas atacamensis]
ATGATCAAGTACATGCTGGATACAAATATCTGCATCTTCACCATCAAGAACAAACCACAAATTGTCCGGGAAGCCTTCAA
TCGACATTCCGGGCAGTTGTGCATCAGCGCTATCACGCTTATGGAGCTTATTTACGGTGCTGAAAAGTCGGCGGCCCCGG
AGAAAAATCTGGGAATTGTTGAAGGATTTGTCGCGCGACTGGATGTCTTGCCTTTCGATAACGACGCAGCCGCACAGGCC
GGGATGGTTCGATCTGAACTCGCGAAGGCTGGAACGCCGATCGGGCCATACGACCAAATGATCGCTGGCCACGCACGCTC
ACTTGGACTCATTGTAGTGACAAACAACGTACGGGAGTTTCAACGCGTTTCCGGTTTGCGAATCGAGGACTGGGTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT160443 WP_003448662.1 NZ_CP054788:c5467448-5467158 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT160443 NZ_CP070503:5716949-5717179 [Pseudomonas atacamensis]
ATGGAGCAGACCACAATTTTCATGAGCAACAGAAGTCAGGCAGTCCGACTGCCAAAGGCAGTTGCGATGCCCGCCGACGT
TAAACGAGTGGAAGTCATCGCGATTGGCAGAACACGCGTCATTACGCCTGCTGGAGAAAGCTGGGATAGCTGGTTTGATG
GCGACAGCGTCAGCCCTGATTTCATGGCTAACCGTGAGCAACCCACTGAACAGGAGCGCGAGTCGTTCTGA

Structures

Toxin

• AlphaFold DB: G8CP73