Detailed information of TA system TA160441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4711584..4712170
• Replicon: chromosome
• Accession: NZ_CP054788
• Organism: Pseudomonas aeruginosa strain YTSY4

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HUK69_RS22235
• Protein ID: WP_003120987.1
• Coordinates: 4711871..4712170 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HUK69_RS22230
• Protein ID: WP_003448662.1
• Coordinates: 4711584..4711874 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK69_RS22215 (HUK69_22185)	4707406..4709382	+	1977	WP_010792226.1	DEAD/DEAH box helicase	-
HUK69_RS31875	4709392..4709526	+	135	WP_033179080.1	hypothetical protein	-
HUK69_RS22220 (HUK69_22190)	4709523..4709816	+	294	WP_049881459.1	hypothetical protein	-
HUK69_RS22225 (HUK69_22195)	4709898..4711169	+	1272	WP_023082545.1	IS4-like element ISPa1635 family transposase	-
HUK69_RS22230 (HUK69_22200)	4711584..4711874	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HUK69_RS22235 (HUK69_22205)	4711871..4712170	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK69_RS22240 (HUK69_22210)	4712372..4713493	+	1122	WP_003448658.1	TcpQ domain-containing protein	-
HUK69_RS22245 (HUK69_22215)	4713493..4715202	+	1710	WP_010792227.1	PilN family type IVB pilus formation outer membrane protein	-
HUK69_RS22250 (HUK69_22220)	4715206..4716531	+	1326	WP_003120992.1	type 4b pilus protein PilO2	-
HUK69_RS22255 (HUK69_22225)	4716521..4717054	+	534	WP_003120993.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4693753..4773470	79717
	-	flank	IS/Tn	-	-	4709898..4711169	1271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T160441 WP_003120987.1 NZ_CP054788:c4712170-4711871 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T160441 NZ_CP070503:5166886-5167137 [Pseudomonas atacamensis]
ATGTCAAAGACTGAAAAACTTCTCGCCAAACTAGTCAACGGCCGAAGCGCATTCACATGGTCCGAGCTTGTCACTCTGTT
GGCGCGTCTTGGTTACTCTCAGCTAGAAGGATCCGGTAGCAGGATCAAATTCGACAACGGGAATCCGAGCGCATTAATCA
ACCTGCACAAGCCACATCCCGGAAATGAAATCAAAACATACGCAAGACGCCAGATTATCGAGCAACTGAAAACAGGAGGC
CTGATTCCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT160441 WP_003448662.1 NZ_CP054788:c4711874-4711584 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT160441 NZ_CP070503:5167134-5167475 [Pseudomonas atacamensis]
ATGAGTTCCCAGCTGCACTACAAAGGCTATGTTGGCTCTATCGAGGCGAGCACGGAAGATAAGTGCCTGTTCGGAAAACT
CCTTTTCATCAGGGCGCTGGTTAATTATGAAGGACAAACGGTGGCTGAACTGGAAGCAGCCTTTCGCGAGGCGGTAGATG
ACTATTTACAAACCTGTGAGCAGCTCGGGCAGAAGCCTGAAATCCCATGCAAAGGATCGTTCAATGTTCGCATCGGTCAC
GATCTGCATTTAGCAGCGGCTCTGGCGGCAACCCGTCAAAATGTGTCGTTGAATGATCTGACACGTCAAGCATTGAACGA
ATACCTCCAACACCACGCATAA

Structures

Toxin

• AlphaFold DB: G8CP73