Detailed information of TA system TA160438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2311955..2312612
• Replicon: chromosome
• Accession: NZ_CP054788
• Organism: Pseudomonas aeruginosa strain YTSY4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8G3IXE4
• Locus tag: HUK69_RS10690
• Protein ID: WP_003098540.1
• Coordinates: 2311955..2312137 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUK69_RS10695
• Protein ID: WP_034013789.1
• Coordinates: 2312184..2312612 (+)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK69_RS10660 (HUK69_10650)	2307000..2308976	-	1977	WP_077563868.1	phage terminase large subunit family protein	-
HUK69_RS10665 (HUK69_10655)	2308948..2309493	-	546	WP_023083814.1	terminase small subunit	-
HUK69_RS10670 (HUK69_10660)	2309613..2310356	-	744	WP_034026712.1	hypothetical protein	-
HUK69_RS10675 (HUK69_10665)	2310353..2310778	-	426	WP_235195803.1	lysis system i-spanin subunit Rz	-
HUK69_RS10680 (HUK69_10670)	2310823..2311440	-	618	WP_121410409.1	glycoside hydrolase family 19 protein	-
HUK69_RS10685 (HUK69_10675)	2311440..2311754	-	315	WP_015649339.1	phage holin, lambda family	-
HUK69_RS10690 (HUK69_10680)	2311955..2312137	+	183	WP_003098540.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUK69_RS10695 (HUK69_10685)	2312184..2312612	+	429	WP_034013789.1	transcriptional regulator	Antitoxin
HUK69_RS10700 (HUK69_10690)	2312729..2313403	-	675	WP_034013787.1	hypothetical protein	-
HUK69_RS10705 (HUK69_10695)	2313441..2313650	-	210	WP_185988403.1	hypothetical protein	-
HUK69_RS10710 (HUK69_10700)	2313734..2314312	-	579	WP_034013783.1	recombination protein NinG	-
HUK69_RS10715 (HUK69_10705)	2314309..2314515	-	207	WP_003159465.1	hypothetical protein	-
HUK69_RS10720 (HUK69_10710)	2314512..2315195	-	684	WP_235195804.1	replication protein P	-
HUK69_RS10725 (HUK69_10715)	2315192..2315947	-	756	WP_235195805.1	helix-turn-helix domain-containing protein	-
HUK69_RS10730 (HUK69_10720)	2316544..2317299	+	756	WP_114231215.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2286591..2331912	45321

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-78)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6746.86 Da
• Isoelectric Point: 11.0775

>T160438 WP_003098540.1 NZ_CP054788:2311955-2312137 [Pseudomonas aeruginosa]
MKFSEFRRWLKAQGVTFEAGKGSHFKITAPNGKQTTFADHGAKEMPEPTRKAIIKQLGLK

Nucleotide

• Length: 183 bp

>T160438 NZ_CP070503:c2881201-2880911 [Pseudomonas atacamensis]
ATGGCTGAATACCGACTTACCCCCGCCGCCGAAGCGGATCTTGAGGCTATCTGGACCTATACAGCTCAACAATGGGGCGT
GGATCAGGCCAATCGCTACATCGACATCCTGACTGCGTCTTTCGAGCAATTGGCTGATCGTCCCAAAACAGCACCCGCAT
GCGACGCGATCCGCCCCGGTTACCGACGCTGCAGCGTGGAGAGTCACGTGATTTATTTTCGAATCACCGCCTATGGGATC
GCGATAATCAGAATCTTGCATGACCGGATGGAAGCGCAGCGTAATCTCTGA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15799.02 Da
• Isoelectric Point: 4.6562

>AT160438 WP_034013789.1 NZ_CP054788:2312184-2312612 [Pseudomonas aeruginosa]
MYDYAIRFEQDDSAPGVAVFCRDLPELNSYGDDKAHAISEAVDAIESTLSLYVDQRREIPAASQAQPGERVIHLPAVTVA
KVALWNEMIRRDMRKADLCRLLGIAQIQGDRLVDFLHNTKMEAMENALSALGLRLSVNIEVA

Nucleotide

• Length: 429 bp

>AT160438 NZ_CP070503:c2881439-2881194 [Pseudomonas atacamensis]
ATGGCTACCGTACGAAAAACCATAACCGTGACTGACCAGCAAGACCATTGGATCAAAGCTCAAATAGAAGCGGGACATTA
CACCAACGACAGCGAATATATCCGCGATTTGATCCGTCGAGAGCAAGAGCGAAACGCTGAACTGGAAACCGTCCGCGCAG
CCCTTCGAGAAGGAGAATCCAGTGGTGCCCCCCGGCCATTCGATGCTGATGCTTTCAAAAAGAGAATGTTGAGGGCGCAT
GGCTGA