Detailed information of TA system TA160437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 2011312..2012820
• Replicon: chromosome
• Accession: NZ_CP054788
• Organism: Pseudomonas aeruginosa strain YTSY4

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX1
• Locus tag: HUK69_RS09245
• Protein ID: WP_003119682.1
• Coordinates: 2012233..2012820 (-)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A9E8LAI1
• Locus tag: HUK69_RS09240
• Protein ID: WP_003117606.1
• Coordinates: 2011312..2012166 (-)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK69_RS09225 (HUK69_09220)	2006731..2006940	+	210	WP_003091604.1	cold-shock protein	-
HUK69_RS09230 (HUK69_09225)	2007001..2008875	-	1875	WP_016562131.1	MFS transporter	-
HUK69_RS09235 (HUK69_09230)	2009050..2011215	-	2166	WP_003115567.1	TonB-dependent receptor family protein	-
HUK69_RS09240 (HUK69_09235)	2011312..2012166	-	855	WP_003117606.1	AraC family transcriptional regulator	Antitoxin
HUK69_RS09245 (HUK69_09240)	2012233..2012820	-	588	WP_003119682.1	GNAT family protein	Toxin
HUK69_RS09250 (HUK69_09245)	2013086..2016565	+	3480	WP_235195781.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21901.23 Da
• Isoelectric Point: 9.8676

>T160437 WP_003119682.1 NZ_CP054788:c2012820-2012233 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T160437 NZ_CP070503:c2697866-2697597 [Pseudomonas atacamensis]
CTGCCGGTTAGCTGGAGTAAACCGGCTCTTGGAGATCTGGCTGACATCATCGAATACGTAGAACAACGCAACACTGTCGC
TTCAGTTGCACTGCACCAAAAGCTCAGCGCCGCAGCGCGAAGGCTTTCCTGGATACCGCATGGTTTCCGCTCCGGCCGAA
TACCGGGCACTCGCGAAATGGTCATCAACTCGAATTATTTACTGGTCTATCGGGTGACAGACCACATCAAAATTCTGACC
TTGTTGCACGCCCGTAAAAAATATCCATAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31863.14 Da
• Isoelectric Point: 7.5295

>AT160437 WP_003117606.1 NZ_CP054788:c2012166-2011312 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Nucleotide

• Length: 855 bp

>AT160437 NZ_CP070503:c2698050-2697856 [Pseudomonas atacamensis]
ATGAGCGCTGAACTTTCACTTATCGTTTCAGACTTCGAAACCGAAGAAGAGGCTGCGGATTACGACCTTTGGTATCGCGC
CAAGGTACAAGCCGCACTTGATGATCCACGCCCGCCTATCCCCCATGATGAGGCAATGGTGCTGCTAGGTCAGATGCTGG
AAGAGAGACGCAAGAATCGACGCGCTGCCGGTTAG

Structures

Toxin

• PDB: 1YRE
• PDB: 8GXJ