Detailed information of TA system TA160436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 144820..145325
• Replicon: chromosome
• Accession: NZ_CP054788
• Organism: Pseudomonas aeruginosa strain YTSY4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: HUK69_RS00675
• Protein ID: WP_003083773.1
• Coordinates: 144820..145101 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: HUK69_RS00680
• Protein ID: WP_003083775.1
• Coordinates: 145098..145325 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK69_RS00650 (HUK69_00650)	140072..141421	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
HUK69_RS00655 (HUK69_00655)	141470..142156	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
HUK69_RS00660 (HUK69_00660)	142256..142990	+	735	WP_003117208.1	GntR family transcriptional regulator	-
HUK69_RS00665 (HUK69_00665)	143170..143580	+	411	WP_003101225.1	aegerolysin family protein	-
HUK69_RS00670 (HUK69_00670)	143612..144520	-	909	WP_003083769.1	LysR family transcriptional regulator	-
HUK69_RS00675 (HUK69_00675)	144820..145101	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
HUK69_RS00680 (HUK69_00680)	145098..145325	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
HUK69_RS00685 (HUK69_00685)	145501..146121	-	621	WP_003101226.1	hypothetical protein	-
HUK69_RS00690 (HUK69_00690)	146222..146722	+	501	WP_003101228.1	LEA type 2 family protein	-
HUK69_RS00695 (HUK69_00695)	146795..147136	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
HUK69_RS00700 (HUK69_00700)	147218..148645	-	1428	WP_003083784.1	GABA permease	-
HUK69_RS00705 (HUK69_00705)	148814..150307	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T160436 WP_003083773.1 NZ_CP054788:c145101-144820 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T160436 NZ_CP070502:c1876797-1876447 [Sporosarcina sp. Marseille-Q4063]
TTGGCAATAAAACGTGGAGACGTCTTTTTTGCAGATCTGTCGCCAGTCGTGGGTTCTGAACAGGGCGGAACTAGACCTGT
TCTTGTAATCCAAAACGATATTGGAAACCGATTTAGCCCGACAGTTATCATTGCGGCTATTACAGCGCAAATTCAAAAAG
CAAAATTGCCGACACATGTTGAGATCGATGCGGAACGCTATCATTTCGAGCGGGATTCGGTGATCCTGCTCGAACAAGTC
CGTACAATCGACAAGTCGAGGCTAACCGATAGAATCACACATCTAGATGAATTTTTGATGGAACAGGTCGATGAAGCACT
AGAAGTGAGTTTCGGCCTCGTCAAATTTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT160436 WP_003083775.1 NZ_CP054788:c145325-145098 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT160436 NZ_CP070502:c1877083-1876802 [Sporosarcina sp. Marseille-Q4063]
TTGCGTGCAAAAAAGAGCATGAGAGAAGTCATTGTAAAGATTCCGAAACGAATGTTGAATGAAAATGAACATACGGTCGT
CCATCAGGAGCCAGAGCGTGGTGGTTTTGTTTATATTTCGACAAAAAGATATGAATCAGATTACGAGCCGGATACGATTC
GCGAAGCGATGATAAAGGGTTATGTCGAAATGTCGCAAATCAACTTGAAAATTGCCACGGAATGCTTACATGTGGAGTTT
GAAGCCCAGCATACGATGGAACGTCTCGTTAGCGGAGGATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9