Detailed information of TA system TA160434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5500109..5500695
• Replicon: chromosome
• Accession: NZ_CP054787
• Organism: Pseudomonas aeruginosa strain HB2011305RE

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HUK67_RS26075
• Protein ID: WP_003120987.1
• Coordinates: 5500396..5500695 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HUK67_RS26070
• Protein ID: WP_003448662.1
• Coordinates: 5500109..5500399 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK67_RS26055 (HUK67_26045)	5495931..5497907	+	1977	WP_010792226.1	DEAD/DEAH box helicase	-
HUK67_RS32490	5497917..5498051	+	135	WP_033179080.1	hypothetical protein	-
HUK67_RS26060 (HUK67_26050)	5498048..5498341	+	294	WP_049881459.1	hypothetical protein	-
HUK67_RS26065 (HUK67_26055)	5498423..5499694	+	1272	WP_023082545.1	IS4-like element ISPa1635 family transposase	-
HUK67_RS26070 (HUK67_26060)	5500109..5500399	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HUK67_RS26075 (HUK67_26065)	5500396..5500695	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK67_RS26080 (HUK67_26070)	5500897..5502018	+	1122	WP_003448658.1	TcpQ domain-containing protein	-
HUK67_RS26085 (HUK67_26075)	5502018..5503727	+	1710	WP_010792227.1	PilN family type IVB pilus formation outer membrane protein	-
HUK67_RS26090 (HUK67_26080)	5503731..5505056	+	1326	WP_003120992.1	type 4b pilus protein PilO2	-
HUK67_RS26095 (HUK67_26085)	5505046..5505579	+	534	WP_003120993.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5470152..5560332	90180
	-	flank	IS/Tn	-	-	5498423..5499694	1271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T160434 WP_003120987.1 NZ_CP054787:c5500695-5500396 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T160434 NZ_CP070501:c1589851-1589414 [Agrococcus sp. Marseille-Q4369]
GTGATCCTGCTCGACACGAGCGTGCTCGTCTACGCCGTCGGCGCAGAGCATCCGCTGCGCGAGCCGTGCCGAGCCGTCAT
CGCCGCCGTCGGCGACGGCACGCTCGCGGCCACGACGACGGTGGAGGTGCTGCAGGAGTTCGCTCATGTGCGTGCTCGGC
GGCGCGGGCGCGACGACGCCGCGGCGCTGACCGAGCGCTACGCGACCGCCTTGGCTCCGCTGATCAGCGTCGACGCAGAC
GATCTCTCGGCCGGGCTGCGCCTCTTCCGGGAGCACGAGCCGCTCGGAGCCTTCGATGCCGTGCTCGCGGCCGTTGCTCA
GCGCCGCGACCACGTGCGAGCGCTCGTCTCCGCGGATCGCGGCTTCGCGAGCATCCCGGGCCTGGTGCACCTCGACCCGG
GCGCGAGCGGCTTCCGCGCCGCGGCCGGCATCGGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT160434 WP_003448662.1 NZ_CP054787:c5500399-5500109 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT160434 NZ_CP070501:c1590069-1589848 [Agrococcus sp. Marseille-Q4369]
GTGCTGATCGAGACCGAGCAGCGGGTGCGGCTTGAGCAGCGGGCGGCCGACCGCGGCGTCTCGGTGGCTGCCCTGGTCAG
GGACGCGATCGACCTGGTGTACCCGCCGCACGGTGATCGCCGCGCCGCCGCGGCAGCCGACATCCTCGCGGCCGAGCCCA
TGGCCGTCGGCGATCCGGCGGCGCTCCGCGCAGAGCTCGACGAGCTCCGGGGCGGCGCGTGA

Structures

Toxin

• AlphaFold DB: G8CP73