Detailed information of TA system TA160432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3596945..3598166
• Replicon: chromosome
• Accession: NZ_CP054787
• Organism: Pseudomonas aeruginosa strain HB2011305RE

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: HUK67_RS16825
• Protein ID: WP_003110488.1
• Coordinates: 3597588..3598166 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUK67_RS16820
• Protein ID: WP_003088112.1
• Coordinates: 3596945..3597499 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK67_RS16795 (HUK67_16780)	3592513..3593064	+	552	WP_003450159.1	XRE family transcriptional regulator	-
HUK67_RS16800 (HUK67_16785)	3593061..3593459	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HUK67_RS16805 (HUK67_16790)	3593472..3593795	-	324	WP_015648646.1	multidrug efflux SMR transporter	-
HUK67_RS16810 (HUK67_16795)	3594126..3594587	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HUK67_RS16815 (HUK67_16800)	3594592..3596787	+	2196	WP_003122807.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HUK67_RS16820 (HUK67_16805)	3596945..3597499	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
HUK67_RS16825 (HUK67_16810)	3597588..3598166	+	579	WP_003110488.1	HD domain-containing protein	Toxin
HUK67_RS16830 (HUK67_16815)	3598275..3599462	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HUK67_RS16835 (HUK67_16820)	3599452..3601623	-	2172	WP_025297367.1	type I secretion system permease/ATPase	-
HUK67_RS16840 (HUK67_16825)	3601613..3602890	-	1278	WP_198580692.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T160432 WP_003110488.1 NZ_CP054787:3597588-3598166 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T160432 NZ_CP070499:c5569633-5569187 [Natronosporangium hydrolyticum]
GTGAGTCTCCTGGCCGACGTCGGTGTCTGGCTGGCCGCCGTCTGGGGCAGGCACGTCCACCACCCGGTTGCCGCGAGCTG
GTTCGAGCAGCAACCGGGTGGGGTCATCCTGTGCCGGGTCACCCAGATGAGTCTGCTCCGGTTGCTGTCCAACCCGGCGG
TTATGGGCGCAGACGCCACCAGTCGCCGTGATGCGTGGGCGGTCGTCGACCGGCTTCGAGCCGATGACCGGGTGTCCTGG
GCAGACGAGCCACTTCAGCTTGAACACGTCTGGCGGGCGATCTCTGCCCGTGACGACGGTAGCCACAAGCTGTGGACCGA
CGACTATCTTGCCGCGTTTGCGCAGGCCGCCGACCTCAGCCTGGTAACGCTCGACCGTCAACTCGCTCGCCGCTACCCCT
CGGTGCGGGTGGAGACGCTCGGCCCATCCCTGACCGCCGCAAACTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT160432 WP_003088112.1 NZ_CP054787:3596945-3597499 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT160432 NZ_CP070499:c5569884-5569630 [Natronosporangium hydrolyticum]
ATGCGCACCACGATTGATCTACCCGCTGAGGTCATGCAGGCGGCGAAGATCCGGGCAGCAGAGCTCGGGGTGACTCTCAA
AGAGCTCTTCAGTCGCGCCATCACCCACGAGCTCGGCTTGCCCGCTGCTCGGCAAGGGGCTAGCCGAGTAACCCTTCCGC
TGGTCGGAGTCGCCGCCACACCAGCGGTCCACGTGACGAACGCCGACATCGAAGCCGCGCTGGCGGCGGACGAAGCGGAG
AGGTACGGCGGGTGA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4