Detailed information of TA system TA160426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 4628539..4629044
• Replicon: chromosome
• Accession: NZ_CP054786
• Organism: Pseudomonas aeruginosa strain DL201330

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: HUK64_RS21475
• Protein ID: WP_003083773.1
• Coordinates: 4628539..4628820 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: HUK64_RS21480
• Protein ID: WP_003083775.1
• Coordinates: 4628817..4629044 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK64_RS21450 (HUK64_21430)	4623790..4625139	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
HUK64_RS21455 (HUK64_21435)	4625188..4625874	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
HUK64_RS21460 (HUK64_21440)	4625975..4626709	+	735	WP_004346926.1	GntR family transcriptional regulator	-
HUK64_RS21465 (HUK64_21445)	4626889..4627299	+	411	WP_003101225.1	aegerolysin family protein	-
HUK64_RS21470 (HUK64_21450)	4627331..4628239	-	909	WP_003083769.1	LysR family transcriptional regulator	-
HUK64_RS21475 (HUK64_21455)	4628539..4628820	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
HUK64_RS21480 (HUK64_21460)	4628817..4629044	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
HUK64_RS21485 (HUK64_21465)	4629220..4629840	-	621	WP_003101226.1	hypothetical protein	-
HUK64_RS21490 (HUK64_21470)	4629941..4630441	+	501	WP_003101228.1	LEA type 2 family protein	-
HUK64_RS21495 (HUK64_21475)	4630514..4630855	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
HUK64_RS21500 (HUK64_21480)	4630937..4632364	-	1428	WP_003083784.1	GABA permease	-
HUK64_RS21505 (HUK64_21485)	4632533..4634026	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T160426 WP_003083773.1 NZ_CP054786:c4628820-4628539 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T160426 NZ_CP070499:c3222261-3221836 [Natronosporangium hydrolyticum]
GTGATCATCCTTGACACGAACGTCATTTCCGAACCACTGCGTCCCCGGCCGGCCGCGGGTGTCGTCACCTGGCTGGACGC
TCAGGCGGTCGAGACCCTCTACCTCACGACGATCAGCCTGGCCGAGGTGCGTTACGGCATCGCCTGCCTACCCGAGGGGC
GACGCCGGCAGGAACTGCAGGATCGGTTCGAGGGCGAGTTCGTGCCGCTGTTCCACGGGCGGACCCTGGCCTTCGACGAG
CCGGCCAGCACCGCCTACGGCACCTTGCGGGCTCAGGCCCGCGCCCAAGGCCTGGCAGTCGGTGACTTCGACGCGCTGAT
CGCCAGTATCGCCCGAGTCACGGGATTCGCAGTCGCCACCCGGGACACCGCACCGTTCGAAGCCACCGGAGTCGCCGTAG
TCAACCCGTTCGACGAGTCGCAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT160426 WP_003083775.1 NZ_CP054786:c4629044-4628817 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT160426 NZ_CP070499:c3222497-3222258 [Natronosporangium hydrolyticum]
GTGGCTGCGATCACTATCCGCAACTTGTCCGACGCGACCCATCGCGCACTGAAGGCCCGCGCGGCCGCGAGCCGCCGCAG
CACTGAGGCTGAGGTGCGCGCGATCCTCGATGCTGCGGTGTCGTCCCCGGACCGGGTCAAGGTAGGTTCCCTGCTGGCGT
CCATCGGCCAGGGGGCGGGGGGCATAGAGCTGGCGGTCACGCGCGAGAAGACCAGCCGCGAGCCACTGGACCTTGCGTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9