Detailed information of TA system TA160424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 2548144..2549402
• Replicon: chromosome
• Accession: NZ_CP054786
• Organism: Pseudomonas aeruginosa strain DL201330

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: HUK64_RS11885
• Protein ID: WP_003086331.1
• Coordinates: 2548144..2548899 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HUK64_RS11890
• Protein ID: WP_003086329.1
• Coordinates: 2549034..2549402 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK64_RS11875 (HUK64_11850)	2543315..2545858	+	2544	WP_033979231.1	acyl-homoserine-lactone acylase QuiP	-
HUK64_RS11880 (HUK64_11855)	2546014..2547375	-	1362	WP_003120632.1	DNA recombination protein RmuC	-
HUK64_RS11885 (HUK64_11860)	2548144..2548899	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
HUK64_RS11890 (HUK64_11865)	2549034..2549402	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HUK64_RS11895 (HUK64_11870)	2549475..2550761	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
HUK64_RS11900 (HUK64_11875)	2550817..2552406	-	1590	WP_023464367.1	aldehyde dehydrogenase family protein	-
HUK64_RS11905 (HUK64_11880)	2552485..2552961	+	477	WP_004353334.1	elongation factor GreAB	-
HUK64_RS11910 (HUK64_11885)	2552964..2553239	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T160424 WP_003086331.1 NZ_CP054786:c2548899-2548144 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T160424 NZ_CP070499:c1719993-1719592 [Natronosporangium hydrolyticum]
GTGAGCGTCTACTACCTCGACACCTCCGCGGCGCTCAAGCTCCTAGCCGAAGAGAGCCATTCCCGCGCATTCGCGACCTT
CTATGATGACCATGCTAACGCGTCATGGGTCAGCTCGGCGCTGCTCCAGATCGAGGTGATCCGCGCCGTCACCAGAGTCA
TGCCGGCCGTGCTACCCGACGCAAGGGACCTCCTGTCGGCGTTCGACTACGTGGCCATCGATGACGAGATCGTCGATGCC
GCCATGAGCGAACCCGACCGAGGGTTGCGTTCACTGGATGCGATCCACCTCGCGACCGCCCGGGCGCTCGACCCCGACCT
GACCGGCGTGGTCACCTACGGCGACCGGTTAGCACGGGCGGCGCAGGACGCCGGCATGACGGTGTTCGCCCCCCAAGACT
GA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT160424 WP_003086329.1 NZ_CP054786:c2549402-2549034 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT160424 NZ_CP070499:c1720286-1719990 [Natronosporangium hydrolyticum]
ATGCCGACGACTAGCGACCACTCGGTTGGCCTGCGGGAGCTACGCCACCACACGAGTGAGGTGCTGGCTCGGGTGCGACA
CGGGGAGACCATCGACGTGACCGAGTACGGGCGGCTGGTTGCTCGGATCGTGCCGGTCGAGCAGCGGGAGCCCGCGCCGG
TGCTGGACCGCATGATCTCCGCTGGTCGGGTGCGGTTGGCGACCCGCCCTGGTTACCGGCCCCGGATGCGGCCCGCGAGC
GGCGCGGATCGACTCGGCGACGCCCTTGCCGATCTCCGCGCCGAGGAACGCTGGTGA

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7