Detailed information of TA system TA160423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 1477168..1478389
• Replicon: chromosome
• Accession: NZ_CP054786
• Organism: Pseudomonas aeruginosa strain DL201330

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: HUK64_RS06635
• Protein ID: WP_003110488.1
• Coordinates: 1477811..1478389 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUK64_RS06630
• Protein ID: WP_003088112.1
• Coordinates: 1477168..1477722 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK64_RS06605 (HUK64_06585)	1472736..1473287	+	552	WP_003450159.1	XRE family transcriptional regulator	-
HUK64_RS06610 (HUK64_06590)	1473284..1473682	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HUK64_RS06615 (HUK64_06595)	1473695..1474018	-	324	WP_015648646.1	multidrug efflux SMR transporter	-
HUK64_RS06620 (HUK64_06600)	1474349..1474810	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HUK64_RS06625 (HUK64_06605)	1474815..1477010	+	2196	WP_003122807.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HUK64_RS06630 (HUK64_06610)	1477168..1477722	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
HUK64_RS06635 (HUK64_06615)	1477811..1478389	+	579	WP_003110488.1	HD domain-containing protein	Toxin
HUK64_RS06640 (HUK64_06620)	1478498..1479685	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HUK64_RS06645 (HUK64_06625)	1479675..1481846	-	2172	WP_025297367.1	type I secretion system permease/ATPase	-
HUK64_RS06650 (HUK64_06630)	1481836..1483113	-	1278	WP_198580692.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T160423 WP_003110488.1 NZ_CP054786:1477811-1478389 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T160423 NZ_CP070499:1646375-1646806 [Natronosporangium hydrolyticum]
ATGCTGGTTGACGCGAACCTGCTGTTGTATGCGGTTGACACCCGCAGCCCGTTCCATGACCGGGCGCGGGAGTGGTTGAC
CGGAGCGCTGAATGGTCGGGAGCGAGTGGGGCTCCCGTGGCAGTGTCTGACCGCGTTCGTGCGCATCGCCACCCACCCCC
GCGCTGCCCAACGGCCGCTCACCCCGGCTCAGGCGTGGGAGCACGTCAGTAGCTGGCTCGCCTGCGAGGTGGCCTGGACC
CCCTTGCCGACCAACCACCACGCCGAGGTGTTAGGTGCCCTGGTGGTGGAGCATGACCTACGCGGCAACCTGGTCAGCGA
CGCCCACCTGGCCGCGTTGGCCATCGAGCACGGCCTCAAGCTCTACTCCGCCGACAGTGACTTTGCGAGGTTCCCGAAGC
TGACCTGGGTGAACCCGTTGGTCAGCGGCTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT160423 WP_003088112.1 NZ_CP054786:1477168-1477722 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT160423 NZ_CP070499:1646145-1646375 [Natronosporangium hydrolyticum]
ATGCGGACTACGATCACCTTCGACGATGACACTGCGGCCGCGGTCGACCAACTGCGGCGCAGCGGCGCGCGAGGTGTCAG
CCAGATCGTCAACGACCTGATCCGGGCTGGGCTCCGTCACCGGGAAGAGCGCCCACCGTTCGTCCAGTCGACCTCGGATC
TGGGGATCCAGGTGGACGTCGCCAACGTCGCGGAGGCGCTCGACCTGCTCGACGGCCCGGACCACCGCTGA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4