160387

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 119870..120540 Replicon plasmid pKP20194a2-p1
Accession NZ_CP054775
Organism Klebsiella pneumoniae strain KP20194a2

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag HUR99_RS27520 Protein ID WP_004213072.1
Coordinates 119870..120313 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag HUR99_RS27525 Protein ID WP_004213073.1
Coordinates 120310..120540 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUR99_RS27485 115281..115556 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
HUR99_RS27490 115619..116110 + 492 WP_011251327.1 DM13 domain-containing protein -
HUR99_RS27495 116159..117079 + 921 WP_004213934.1 DUF1471 domain-containing protein -
HUR99_RS27500 117170..117576 + 407 Protein_132 GAF domain-containing protein -
HUR99_RS27505 118091..118726 - 636 Protein_133 response regulator -
HUR99_RS27510 119143..119436 + 294 Protein_134 transposase -
HUR99_RS27515 119440..119856 + 417 Protein_135 restriction endonuclease -
HUR99_RS27520 119870..120313 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
HUR99_RS27525 120310..120540 - 231 WP_004213073.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HUR99_RS27530 121148..122281 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
HUR99_RS27535 122297..122590 + 294 WP_004213076.1 hypothetical protein -
HUR99_RS27540 122580..122786 - 207 WP_004213077.1 hypothetical protein -
HUR99_RS27545 123138..123428 + 291 WP_004213078.1 hypothetical protein -
HUR99_RS27550 123418..124317 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..195030 195030


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T160387 WP_004213072.1 NZ_CP054775:c120313-119870 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T160387 NZ_CP070482:3661486-3661593 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT160387 WP_004213073.1 NZ_CP054775:c120540-120310 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT160387 NZ_CP070482:c3661437-3661371 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6U619

Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References