Detailed information of TA system TA160386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 79799..80709
• Replicon: plasmid pKP20194a2-p1
• Accession: NZ_CP054775
• Organism: Klebsiella pneumoniae strain KP20194a2

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: HUR99_RS27280
• Protein ID: WP_004026354.1
• Coordinates: 79799..80269 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: HUR99_RS27285
• Protein ID: WP_004026357.1
• Coordinates: 80266..80709 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR99_RS27240	75115..75429	+	315	WP_011251273.1	hypothetical protein	-
HUR99_RS27245	75438..75948	+	511	Protein_81	hypothetical protein	-
HUR99_RS27250	75938..76378	+	441	WP_011251275.1	hypothetical protein	-
HUR99_RS27255	76803..77759	-	957	WP_011251280.1	DsbA family protein	-
HUR99_RS27260	77819..78160	-	342	WP_011251281.1	hypothetical protein	-
HUR99_RS27265	78174..78485	-	312	WP_011251282.1	hypothetical protein	-
HUR99_RS27270	78502..78936	-	435	Protein_86	thioredoxin fold domain-containing protein	-
HUR99_RS27275	78953..79650	-	698	Protein_87	IS1 family transposase	-
HUR99_RS27280	79799..80269	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
HUR99_RS27285	80266..80709	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
HUR99_RS27290	80810..81313	-	504	Protein_90	DUF4113 domain-containing protein	-
HUR99_RS27295	81519..82499	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
HUR99_RS27300	82565..83872	+	1308	Protein_92	FepA family TonB-dependent siderophore receptor	-
HUR99_RS27305	83934..84155	+	222	WP_004213615.1	hypothetical protein	-
HUR99_RS27310	84298..85200	-	903	WP_004213613.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..195030	195030
	-	inside	IScluster/Tn	-	iroN / rmpA	73518..93494	19976

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T160386 WP_004026354.1 NZ_CP054775:c80269-79799 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T160386 NZ_CP070482:c3007010-3006837 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT160386 WP_004026357.1 NZ_CP054775:c80709-80266 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT160386 NZ_CP070482:c3007334-3007038 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9