Detailed information of TA system TA160357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 119873..120543
• Replicon: plasmid pKP20194b-p1
• Accession: NZ_CP054769
• Organism: Klebsiella pneumoniae strain KP20194b

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HUS00_RS27520
• Protein ID: WP_004213072.1
• Coordinates: 119873..120316 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HUS00_RS27525
• Protein ID: WP_004213073.1
• Coordinates: 120313..120543 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS00_RS27485	115284..115559	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
HUS00_RS27490	115622..116113	+	492	WP_011251327.1	DM13 domain-containing protein	-
HUS00_RS27495	116162..117082	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
HUS00_RS27500	117173..117579	+	407	Protein_132	GAF domain-containing protein	-
HUS00_RS27505	118094..118729	-	636	Protein_133	response regulator	-
HUS00_RS27510	119146..119439	+	294	Protein_134	transposase	-
HUS00_RS27515	119443..119859	+	417	Protein_135	restriction endonuclease	-
HUS00_RS27520	119873..120316	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUS00_RS27525	120313..120543	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HUS00_RS27530	121151..122284	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HUS00_RS27535	122300..122593	+	294	WP_004213076.1	hypothetical protein	-
HUS00_RS27540	122583..122789	-	207	WP_004213077.1	hypothetical protein	-
HUS00_RS27545	123141..123431	+	291	WP_004213078.1	hypothetical protein	-
HUS00_RS27550	123421..124320	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..195034	195034

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T160357 WP_004213072.1 NZ_CP054769:c120316-119873 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T160357 NZ_CP070471:c271054-270773 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTGGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGAACCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT160357 WP_004213073.1 NZ_CP054769:c120543-120313 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT160357 NZ_CP070471:c271278-271051 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620