Detailed information of TA system TA160356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 79802..80712
• Replicon: plasmid pKP20194b-p1
• Accession: NZ_CP054769
• Organism: Klebsiella pneumoniae strain KP20194b

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: HUS00_RS27280
• Protein ID: WP_004026354.1
• Coordinates: 79802..80272 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: HUS00_RS27285
• Protein ID: WP_004026357.1
• Coordinates: 80269..80712 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS00_RS27240	75118..75432	+	315	WP_011251273.1	hypothetical protein	-
HUS00_RS27245	75441..75951	+	511	Protein_81	hypothetical protein	-
HUS00_RS27250	75941..76381	+	441	WP_011251275.1	hypothetical protein	-
HUS00_RS27255	76806..77762	-	957	WP_011251280.1	DsbA family protein	-
HUS00_RS27260	77822..78163	-	342	WP_011251281.1	hypothetical protein	-
HUS00_RS27265	78177..78488	-	312	WP_011251282.1	hypothetical protein	-
HUS00_RS27270	78505..78939	-	435	Protein_86	thioredoxin fold domain-containing protein	-
HUS00_RS27275	78956..79653	-	698	Protein_87	IS1 family transposase	-
HUS00_RS27280	79802..80272	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
HUS00_RS27285	80269..80712	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
HUS00_RS27290	80813..81316	-	504	Protein_90	DUF4113 domain-containing protein	-
HUS00_RS27295	81522..82502	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
HUS00_RS27300	82568..83875	+	1308	Protein_92	FepA family TonB-dependent siderophore receptor	-
HUS00_RS27305	83937..84158	+	222	WP_004213615.1	hypothetical protein	-
HUS00_RS27310	84301..85203	-	903	WP_004213613.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..195034	195034
	-	inside	IScluster/Tn	-	iroN / rmpA	73521..93497	19976

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T160356 WP_004026354.1 NZ_CP054769:c80272-79802 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T160356 NZ_CP070471:c178600-178307 [Pseudomonas aeruginosa]
ATGCCACAAGTGATCCTCTCTCCGGCTGCTCTGCGTGACCTTAAACGGCTGCGCGAGTTCTTGCACAGTAAGAGCCCAGT
TGTTGCACAGCGAGCAGCCCAAACCATCCGTTCGGCGCTGGCCCAGCTCGGGCAACAACCCGCGATGGGGCGTCCTATTG
AAGGGCTCCCGGAGGTGTTCCGCGAGTGGGTCATCCCCTTTGGGGACAGTGGTTATCTGGCGCGTTACCGCCTAGAGGGC
GAAACCGTAGTGATCTTGGCTCTGCGTCACCAGCGCGAAGCCGGGTTTATTTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT160356 WP_004026357.1 NZ_CP054769:c80712-80269 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT160356 NZ_CP070471:c178875-178588 [Pseudomonas aeruginosa]
ATGTCCGCAGCTACCAAACCCATGTCTATCAAACTTGATAGCGATACCCAAAGCCGGATCAAGCAGCTAGCTGCTGCCCG
CGACCGTTCAGCGCACTGGATGATGCGCGAAGCTATCACCCAGTATGTTGAGCGCGAAGAGCGCAAGGAGGCGTTCCGCC
GCGATACCCTCGATGCTTGGCAGGAGTTTCAGGAGACTGGTTTGCATGTCTCTCTTGAGGAGACCGATCAGTGGTTGGCT
GGTTGGGGCACCGAGATTGAAGGTGGTGCACCTGCATGCCACAAGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9