Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 79794..80704 Replicon plasmid pKP20194c-p1
Accession NZ_CP054757
Organism Klebsiella pneumoniae strain KP20194c

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag HUS02_RS27290 Protein ID WP_004026354.1
Coordinates 79794..80264 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag HUS02_RS27295 Protein ID WP_004026357.1
Coordinates 80261..80704 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS02_RS27250 75110..75424 + 315 WP_011251273.1 hypothetical protein -
HUS02_RS27255 75433..75943 + 511 Protein_81 hypothetical protein -
HUS02_RS27260 75933..76373 + 441 WP_011251275.1 hypothetical protein -
HUS02_RS27265 76798..77754 - 957 WP_011251280.1 DsbA family protein -
HUS02_RS27270 77814..78155 - 342 WP_011251281.1 hypothetical protein -
HUS02_RS27275 78169..78480 - 312 WP_011251282.1 hypothetical protein -
HUS02_RS27280 78497..78931 - 435 Protein_86 thioredoxin fold domain-containing protein -
HUS02_RS27285 78948..79645 - 698 Protein_87 IS1 family transposase -
HUS02_RS27290 79794..80264 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
HUS02_RS27295 80261..80704 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
HUS02_RS27300 80805..81308 - 504 Protein_90 DUF4113 domain-containing protein -
HUS02_RS27305 81514..82494 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
HUS02_RS27310 82560..83867 + 1308 Protein_92 FepA family TonB-dependent siderophore receptor -
HUS02_RS27315 83929..84150 + 222 WP_004213615.1 hypothetical protein -
HUS02_RS27320 84293..85195 - 903 WP_004213613.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..195024 195024
- inside IScluster/Tn - iroN / rmpA 73513..93489 19976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T160299 WP_004026354.1 NZ_CP054757:c80264-79794 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T160299 NZ_CP070440:1949634-1949736 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT160299 WP_004026357.1 NZ_CP054757:c80704-80261 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT160299 NZ_CP070440:c1949740-1949593 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References