Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5370312..5370937 Replicon chromosome
Accession NZ_CP054756
Organism Klebsiella pneumoniae strain KP20194c

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUS02_RS26475 Protein ID WP_002882817.1
Coordinates 5370312..5370695 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUS02_RS26480 Protein ID WP_004150355.1
Coordinates 5370695..5370937 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS02_RS26460 5367678..5368580 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUS02_RS26465 5368577..5369212 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUS02_RS26470 5369209..5370138 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUS02_RS26475 5370312..5370695 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUS02_RS26480 5370695..5370937 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HUS02_RS26485 5371142..5372059 + 918 WP_002882812.1 alpha/beta hydrolase -
HUS02_RS26490 5372073..5373014 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUS02_RS26495 5373059..5373496 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HUS02_RS26500 5373493..5374353 - 861 WP_002882807.1 virulence factor BrkB family protein -
HUS02_RS26505 5374347..5374946 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T160296 WP_002882817.1 NZ_CP054756:c5370695-5370312 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T160296 NZ_CP070438:6401-6817 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTTGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCGGCCGGCGCCGTACTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT160296 WP_004150355.1 NZ_CP054756:c5370937-5370695 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT160296 NZ_CP070438:6174-6404 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References