Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4171323..4171942 Replicon chromosome
Accession NZ_CP054756
Organism Klebsiella pneumoniae strain KP20194c

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUS02_RS20730 Protein ID WP_002892050.1
Coordinates 4171724..4171942 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUS02_RS20725 Protein ID WP_002892066.1
Coordinates 4171323..4171697 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS02_RS20715 4166475..4167668 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUS02_RS20720 4167691..4170837 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
HUS02_RS20725 4171323..4171697 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUS02_RS20730 4171724..4171942 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HUS02_RS20735 4172101..4172667 + 567 WP_002892030.1 maltose O-acetyltransferase -
HUS02_RS20740 4172639..4172779 - 141 WP_004147370.1 hypothetical protein -
HUS02_RS20745 4172800..4173270 + 471 WP_002892026.1 YlaC family protein -
HUS02_RS20750 4173245..4174696 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HUS02_RS20755 4174797..4175495 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
HUS02_RS20760 4175492..4175632 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUS02_RS20765 4175632..4175895 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T160292 WP_002892050.1 NZ_CP054756:4171724-4171942 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T160292 NZ_CP070435:c5243688-5243305 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGAAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT160292 WP_002892066.1 NZ_CP054756:4171323-4171697 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT160292 NZ_CP070435:c5243930-5243688 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References