Detailed information of TA system TA160271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 119867..120537
• Replicon: plasmid pKP20194c3-p1
• Accession: NZ_CP054751
• Organism: Klebsiella pneumoniae strain KP20194c3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HUS03_RS27535
• Protein ID: WP_004213072.1
• Coordinates: 119867..120310 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HUS03_RS27540
• Protein ID: WP_004213073.1
• Coordinates: 120307..120537 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS03_RS27500	115278..115553	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
HUS03_RS27505	115616..116107	+	492	WP_011251327.1	DM13 domain-containing protein	-
HUS03_RS27510	116156..117076	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
HUS03_RS27515	117167..117573	+	407	Protein_132	GAF domain-containing protein	-
HUS03_RS27520	118088..118723	-	636	Protein_133	response regulator	-
HUS03_RS27525	119140..119433	+	294	Protein_134	transposase	-
HUS03_RS27530	119437..119853	+	417	Protein_135	restriction endonuclease	-
HUS03_RS27535	119867..120310	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUS03_RS27540	120307..120537	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HUS03_RS27545	121145..122278	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HUS03_RS27550	122294..122587	+	294	WP_004213076.1	hypothetical protein	-
HUS03_RS27555	122577..122783	-	207	WP_004213077.1	hypothetical protein	-
HUS03_RS27560	123135..123425	+	291	WP_004213078.1	hypothetical protein	-
HUS03_RS27565	123415..124314	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..195028	195028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T160271 WP_004213072.1 NZ_CP054751:c120310-119867 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T160271 NZ_CP070425:c4661062-4660529 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGATTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT160271 WP_004213073.1 NZ_CP054751:c120537-120307 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT160271 NZ_CP070425:c4661339-4661073 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620