Detailed information of TA system TA160270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 79796..80706
• Replicon: plasmid pKP20194c3-p1
• Accession: NZ_CP054751
• Organism: Klebsiella pneumoniae strain KP20194c3

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: HUS03_RS27295
• Protein ID: WP_004026354.1
• Coordinates: 79796..80266 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: HUS03_RS27300
• Protein ID: WP_004026357.1
• Coordinates: 80263..80706 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS03_RS27255	75112..75426	+	315	WP_011251273.1	hypothetical protein	-
HUS03_RS27260	75435..75945	+	511	Protein_81	hypothetical protein	-
HUS03_RS27265	75935..76375	+	441	WP_011251275.1	hypothetical protein	-
HUS03_RS27270	76800..77756	-	957	WP_011251280.1	DsbA family protein	-
HUS03_RS27275	77816..78157	-	342	WP_011251281.1	hypothetical protein	-
HUS03_RS27280	78171..78482	-	312	WP_011251282.1	hypothetical protein	-
HUS03_RS27285	78499..78933	-	435	Protein_86	thioredoxin fold domain-containing protein	-
HUS03_RS27290	78950..79647	-	698	Protein_87	IS1 family transposase	-
HUS03_RS27295	79796..80266	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
HUS03_RS27300	80263..80706	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
HUS03_RS27305	80807..81310	-	504	Protein_90	DUF4113 domain-containing protein	-
HUS03_RS27310	81516..82496	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
HUS03_RS27315	82562..83869	+	1308	Protein_92	FepA family TonB-dependent siderophore receptor	-
HUS03_RS27320	83931..84152	+	222	WP_004213615.1	hypothetical protein	-
HUS03_RS27325	84295..85197	-	903	WP_004213613.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..195028	195028
	-	inside	IScluster/Tn	-	iroN / rmpA	73515..93491	19976

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T160270 WP_004026354.1 NZ_CP054751:c80266-79796 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T160270 NZ_CP070425:4057439-4057657 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT160270 WP_004026357.1 NZ_CP054751:c80706-80263 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT160270 NZ_CP070425:4057038-4057412 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9