Detailed information of TA system TA160262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2736399..2737987
• Replicon: chromosome
• Accession: NZ_CP054750
• Organism: Klebsiella pneumoniae strain KP20194c3

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0H3GN97
• Locus tag: HUS03_RS13535
• Protein ID: WP_004199851.1
• Coordinates: 2736399..2737721 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: HUS03_RS13540
• Protein ID: WP_002904790.1
• Coordinates: 2737721..2737987 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS03_RS13520	2731464..2733965	+	2502	WP_004199853.1	fimbrial biogenesis outer membrane usher protein	-
HUS03_RS13525	2733953..2734957	+	1005	WP_004199852.1	type 1 fimbrial protein	-
HUS03_RS13530	2735090..2736382	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
HUS03_RS13535	2736399..2737721	-	1323	WP_004199851.1	type II toxin-antitoxin system HipA family toxin	Toxin
HUS03_RS13540	2737721..2737987	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HUS03_RS13545	2738253..2738579	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
HUS03_RS13550	2738576..2739076	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HUS03_RS13555	2739054..2740433	-	1380	WP_004199850.1	purine permease	-
HUS03_RS13560	2740779..2741933	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
HUS03_RS13565	2741914..2742837	-	924	WP_004199849.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49128.44 Da
• Isoelectric Point: 7.2487

>T160262 WP_004199851.1 NZ_CP054750:c2737721-2736399 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPLEQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T160262 NZ_CP070421:c84728-84312 [Klebsiella quasipneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT160262 WP_002904790.1 NZ_CP054750:c2737987-2737721 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT160262 NZ_CP070421:c84955-84725 [Klebsiella quasipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GN97

Antitoxin

• AlphaFold DB: A0A0H3GXD8