Detailed information of TA system TA160242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 119869..120539
• Replicon: plasmid pKP20194c4-p1
• Accession: NZ_CP054745
• Organism: Klebsiella pneumoniae strain KP20194c4

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HUS04_RS27530
• Protein ID: WP_004213072.1
• Coordinates: 119869..120312 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HUS04_RS27535
• Protein ID: WP_004213073.1
• Coordinates: 120309..120539 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS04_RS27495	115280..115555	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
HUS04_RS27500	115618..116109	+	492	WP_011251327.1	DM13 domain-containing protein	-
HUS04_RS27505	116158..117078	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
HUS04_RS27510	117169..117575	+	407	Protein_132	GAF domain-containing protein	-
HUS04_RS27515	118090..118725	-	636	Protein_133	response regulator	-
HUS04_RS27520	119142..119435	+	294	Protein_134	transposase	-
HUS04_RS27525	119439..119855	+	417	Protein_135	restriction endonuclease	-
HUS04_RS27530	119869..120312	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUS04_RS27535	120309..120539	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HUS04_RS27540	121147..122280	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HUS04_RS27545	122296..122589	+	294	WP_004213076.1	hypothetical protein	-
HUS04_RS27550	122579..122785	-	207	WP_004213077.1	hypothetical protein	-
HUS04_RS27555	123137..123427	+	291	WP_004213078.1	hypothetical protein	-
HUS04_RS27560	123417..124316	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..195025	195025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T160242 WP_004213072.1 NZ_CP054745:c120312-119869 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T160242 NZ_CP070410:c3884136-3883819 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT160242 WP_004213073.1 NZ_CP054745:c120539-120309 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT160242 NZ_CP070410:c3883826-3883539 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620