Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5371329..5371954 Replicon chromosome
Accession NZ_CP054744
Organism Klebsiella pneumoniae strain KP20194c4

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUS04_RS26475 Protein ID WP_002882817.1
Coordinates 5371329..5371712 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUS04_RS26480 Protein ID WP_004150355.1
Coordinates 5371712..5371954 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS04_RS26460 5368695..5369597 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUS04_RS26465 5369594..5370229 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUS04_RS26470 5370226..5371155 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUS04_RS26475 5371329..5371712 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUS04_RS26480 5371712..5371954 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HUS04_RS26485 5372159..5373076 + 918 WP_002882812.1 alpha/beta hydrolase -
HUS04_RS26490 5373090..5374031 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUS04_RS26495 5374076..5374513 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HUS04_RS26500 5374510..5375370 - 861 WP_002882807.1 virulence factor BrkB family protein -
HUS04_RS26505 5375364..5375963 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T160238 WP_002882817.1 NZ_CP054744:c5371712-5371329 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T160238 NZ_CP070410:337164-337532 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT160238 WP_004150355.1 NZ_CP054744:c5371954-5371712 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT160238 NZ_CP070410:336946-337167 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References