160209

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5370493..5371118 Replicon chromosome
Accession NZ_CP054738
Organism Klebsiella pneumoniae strain KP20194c5

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUS05_RS26480 Protein ID WP_002882817.1
Coordinates 5370493..5370876 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUS05_RS26485 Protein ID WP_004150355.1
Coordinates 5370876..5371118 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS05_RS26465 5367859..5368761 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUS05_RS26470 5368758..5369393 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUS05_RS26475 5369390..5370319 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUS05_RS26480 5370493..5370876 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUS05_RS26485 5370876..5371118 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HUS05_RS26490 5371323..5372240 + 918 WP_002882812.1 alpha/beta hydrolase -
HUS05_RS26495 5372254..5373195 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUS05_RS26500 5373240..5373677 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HUS05_RS26505 5373674..5374534 - 861 WP_002882807.1 virulence factor BrkB family protein -
HUS05_RS26510 5374528..5375127 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T160209 WP_002882817.1 NZ_CP054738:c5370876-5370493 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T160209 NZ_CP070338:c3705823-3705422 [Mycobacterium tuberculosis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT160209 WP_004150355.1 NZ_CP054738:c5371118-5370876 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT160209 NZ_CP070338:c3706047-3705820 [Mycobacterium tuberculosis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References