Detailed information of TA system TA160190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 3422..4002
• Replicon: plasmid pKP20194d-p4
• Accession: NZ_CP054736
• Organism: Klebsiella pneumoniae strain KP20194d

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: HUS06_RS29205
• Protein ID: WP_071177730.1
• Coordinates: 3422..3736 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: HUS06_RS29210
• Protein ID: WP_000093040.1
• Coordinates: 3724..4002 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS06_RS29185	1330..2061	+	732	WP_071177728.1	replication-relaxation family protein	-
HUS06_RS29190	2068..2598	+	531	WP_071177729.1	hypothetical protein	-
HUS06_RS29195	2625..2804	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
HUS06_RS29200	2830..3258	-	429	WP_001140599.1	hypothetical protein	-
HUS06_RS29205	3422..3736	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUS06_RS29210	3724..4002	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
HUS06_RS29215	4336..4542	-	207	Protein_7	TonB family protein	-
HUS06_RS29220	4539..4910	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
HUS06_RS29225	5184..5429	-	246	WP_032440458.1	hypothetical protein	-
HUS06_RS29230	6074..7561	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T160190 WP_071177730.1 NZ_CP054736:c3736-3422 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T160190 NZ_CP070338:c2647246-2646851 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT160190 WP_000093040.1 NZ_CP054736:c4002-3724 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT160190 NZ_CP070338:c2647488-2647243 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1