Detailed information of TA system TA160182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 79797..80707
• Replicon: plasmid pKP20194d-p1
• Accession: NZ_CP054733
• Organism: Klebsiella pneumoniae strain KP20194d

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: HUS06_RS27285
• Protein ID: WP_004026354.1
• Coordinates: 79797..80267 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: HUS06_RS27290
• Protein ID: WP_004026357.1
• Coordinates: 80264..80707 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS06_RS27245	75113..75427	+	315	WP_011251273.1	hypothetical protein	-
HUS06_RS27250	75436..75946	+	511	Protein_81	hypothetical protein	-
HUS06_RS27255	75936..76376	+	441	WP_011251275.1	hypothetical protein	-
HUS06_RS27260	76801..77757	-	957	WP_011251280.1	DsbA family protein	-
HUS06_RS27265	77817..78158	-	342	WP_011251281.1	hypothetical protein	-
HUS06_RS27270	78172..78483	-	312	WP_011251282.1	hypothetical protein	-
HUS06_RS27275	78500..78934	-	435	Protein_86	thioredoxin fold domain-containing protein	-
HUS06_RS27280	78951..79648	-	698	Protein_87	IS1 family transposase	-
HUS06_RS27285	79797..80267	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
HUS06_RS27290	80264..80707	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
HUS06_RS27295	80808..81311	-	504	Protein_90	DUF4113 domain-containing protein	-
HUS06_RS27300	81517..82495	-	979	Protein_91	IS5 family transposase	-
HUS06_RS27305	82561..83868	+	1308	Protein_92	FepA family TonB-dependent siderophore receptor	-
HUS06_RS27310	83930..84151	+	222	WP_004213615.1	hypothetical protein	-
HUS06_RS27315	84294..85196	-	903	WP_004213613.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..194896	194896
	-	inside	IScluster/Tn	-	iroN / rmpA	73516..93490	19974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T160182 WP_004026354.1 NZ_CP054733:c80267-79797 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T160182 NZ_CP070338:2029470-2029868 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT160182 WP_004026357.1 NZ_CP054733:c80707-80264 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT160182 NZ_CP070338:2029242-2029469 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9