160179

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5370129..5370754 Replicon chromosome
Accession NZ_CP054732
Organism Klebsiella pneumoniae strain KP20194d

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUS06_RS26470 Protein ID WP_002882817.1
Coordinates 5370129..5370512 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUS06_RS26475 Protein ID WP_004150355.1
Coordinates 5370512..5370754 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS06_RS26455 5367495..5368397 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUS06_RS26460 5368394..5369029 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUS06_RS26465 5369026..5369955 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUS06_RS26470 5370129..5370512 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUS06_RS26475 5370512..5370754 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HUS06_RS26480 5370959..5371876 + 918 WP_002882812.1 alpha/beta hydrolase -
HUS06_RS26485 5371890..5372831 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUS06_RS26490 5372876..5373313 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HUS06_RS26495 5373310..5374170 - 861 WP_002882807.1 virulence factor BrkB family protein -
HUS06_RS26500 5374164..5374763 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T160179 WP_002882817.1 NZ_CP054732:c5370512-5370129 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T160179 NZ_CP070338:c1997031-1996612 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT160179 WP_004150355.1 NZ_CP054732:c5370754-5370512 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT160179 NZ_CP070338:c1997300-1997040 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References