160153

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 119867..120537 Replicon plasmid pKP20194e-p1
Accession NZ_CP054727
Organism Klebsiella pneumoniae strain KP20194e

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag HUS07_RS27530 Protein ID WP_004213072.1
Coordinates 119867..120310 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag HUS07_RS27535 Protein ID WP_004213073.1
Coordinates 120307..120537 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS07_RS27495 115278..115553 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
HUS07_RS27500 115616..116107 + 492 WP_011251327.1 DM13 domain-containing protein -
HUS07_RS27505 116156..117076 + 921 WP_004213934.1 DUF1471 domain-containing protein -
HUS07_RS27510 117167..117573 + 407 Protein_132 GAF domain-containing protein -
HUS07_RS27515 118088..118723 - 636 Protein_133 response regulator -
HUS07_RS27520 119140..119433 + 294 Protein_134 transposase -
HUS07_RS27525 119437..119853 + 417 Protein_135 restriction endonuclease -
HUS07_RS27530 119867..120310 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
HUS07_RS27535 120307..120537 - 231 WP_004213073.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HUS07_RS27540 121145..122278 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
HUS07_RS27545 122294..122587 + 294 WP_004213076.1 hypothetical protein -
HUS07_RS27550 122577..122783 - 207 WP_004213077.1 hypothetical protein -
HUS07_RS27555 123135..123425 + 291 WP_004213078.1 hypothetical protein -
HUS07_RS27560 123415..124314 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..195027 195027


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T160153 WP_004213072.1 NZ_CP054727:c120310-119867 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T160153 NZ_CP070327:c2336208-2336044 [Ferrovum myxofaciens]
ATGAGCGTACTCATCGACACCTCGGTGTGGATAGACCACTTCCGCAACGGAAATGATGCGTTGATTGACTTGATCGGGCT
AGACCTCGCGCTGACTCACCCAATGGTCATACTCGAAATTGCGTGCGGGACCCCCCCCAGCGCCACGCATCCAGACACTG
GATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT160153 WP_004213073.1 NZ_CP054727:c120537-120307 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT160153 NZ_CP070327:c2336411-2336205 [Ferrovum myxofaciens]
ATGCGTACCACTGTCACTATCGACGATGCACTCTACGAAAAGGCCCTGGAAATGGCCGACCCAAATATGGACAAATCGGA
TATTTTCCGAGAAGCAATCAAGACATTCGTGCGCGTGCAGGCAGCCAAGCGTCTTGCAACTCTAGGCGGCATGATACCCA
AGATTCAGGATGTACCGCGTCGCCGTGAGGAGCCAGTCGTGCAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q6U619


Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References