Detailed information of TA system TA160122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 79798..80708
• Replicon: plasmid pKP20194f-p1
• Accession: NZ_CP054721
• Organism: Klebsiella pneumoniae strain KP20194f

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: HUS08_RS27290
• Protein ID: WP_004026354.1
• Coordinates: 79798..80268 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: HUS08_RS27295
• Protein ID: WP_004026357.1
• Coordinates: 80265..80708 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS08_RS27250	75114..75428	+	315	WP_011251273.1	hypothetical protein	-
HUS08_RS27255	75437..75947	+	511	Protein_81	hypothetical protein	-
HUS08_RS27260	75937..76377	+	441	WP_011251275.1	hypothetical protein	-
HUS08_RS27265	76802..77758	-	957	WP_011251280.1	DsbA family protein	-
HUS08_RS27270	77818..78159	-	342	WP_011251281.1	hypothetical protein	-
HUS08_RS27275	78173..78484	-	312	WP_011251282.1	hypothetical protein	-
HUS08_RS27280	78501..78935	-	435	Protein_86	thioredoxin fold domain-containing protein	-
HUS08_RS27285	78952..79649	-	698	Protein_87	IS1 family transposase	-
HUS08_RS27290	79798..80268	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
HUS08_RS27295	80265..80708	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
HUS08_RS27300	80809..81312	-	504	Protein_90	DUF4113 domain-containing protein	-
HUS08_RS27305	81518..82498	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
HUS08_RS27310	82564..83871	+	1308	Protein_92	FepA family TonB-dependent siderophore receptor	-
HUS08_RS27315	83933..84154	+	222	WP_004213615.1	hypothetical protein	-
HUS08_RS27320	84297..85199	-	903	WP_004213613.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..194901	194901
	-	inside	IScluster/Tn	-	iroN / rmpA	73517..93493	19976

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T160122 WP_004026354.1 NZ_CP054721:c80268-79798 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T160122 NZ_CP070298:c12189-11908 [Escherichia albertii]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAAAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT160122 WP_004026357.1 NZ_CP054721:c80708-80265 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT160122 NZ_CP070298:c12430-12179 [Escherichia albertii]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9