Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5370311..5370936 Replicon chromosome
Accession NZ_CP054720
Organism Klebsiella pneumoniae strain KP20194f

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUS08_RS26475 Protein ID WP_002882817.1
Coordinates 5370311..5370694 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUS08_RS26480 Protein ID WP_004150355.1
Coordinates 5370694..5370936 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS08_RS26460 5367677..5368579 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUS08_RS26465 5368576..5369211 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUS08_RS26470 5369208..5370137 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUS08_RS26475 5370311..5370694 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUS08_RS26480 5370694..5370936 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HUS08_RS26485 5371141..5372058 + 918 WP_002882812.1 alpha/beta hydrolase -
HUS08_RS26490 5372072..5373013 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUS08_RS26495 5373058..5373495 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HUS08_RS26500 5373492..5374352 - 861 WP_002882807.1 virulence factor BrkB family protein -
HUS08_RS26505 5374346..5374945 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T160119 WP_002882817.1 NZ_CP054720:c5370694-5370311 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T160119 NZ_CP070296:4058886-4059227 [Escherichia albertii]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGCCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGATAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCCCCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGAATTTATTGGGGTGATTGC
CGGTAAACAGAAAGTCGCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT160119 WP_004150355.1 NZ_CP054720:c5370936-5370694 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT160119 NZ_CP070296:c4058891-4058815 [Escherichia albertii]
AGTCATGATTACTATTCTCCATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References