Detailed information of TA system TA160097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1225161..1226078
• Replicon: chromosome
• Accession: NZ_CP054714
• Organism: Bacillus velezensis strain KKLW

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: HUO02_RS06445
• Protein ID: WP_012117368.1
• Coordinates: 1225332..1226078 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: HUO02_RS06440
• Protein ID: WP_003154807.1
• Coordinates: 1225161..1225331 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUO02_RS06395	1220295..1220807	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
HUO02_RS06400	1220920..1221141	+	222	Protein_1199	terminase	-
HUO02_RS06405	1221550..1222017	+	468	WP_077411063.1	hypothetical protein	-
HUO02_RS06410	1222030..1222401	+	372	WP_012117365.1	hypothetical protein	-
HUO02_RS06415	1222406..1222603	+	198	WP_012117366.1	XkdX family protein	-
HUO02_RS06420	1222660..1223421	+	762	WP_012117367.1	hypothetical protein	-
HUO02_RS06425	1223473..1223736	+	264	WP_003154815.1	hemolysin XhlA family protein	-
HUO02_RS06430	1223750..1224013	+	264	WP_003154813.1	phage holin	-
HUO02_RS06435	1224027..1224905	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
HUO02_RS06440	1225161..1225331	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
HUO02_RS06445	1225332..1226078	-	747	WP_012117368.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HUO02_RS06450	1226183..1227181	-	999	WP_007407255.1	inorganic phosphate transporter	-
HUO02_RS06455	1227194..1227811	-	618	WP_003154804.1	DUF47 domain-containing protein	-
HUO02_RS06460	1228097..1229413	-	1317	WP_007610842.1	amino acid permease	-
HUO02_RS06465	1229736..1230686	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1211229..1283365	72136

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29119.61 Da
• Isoelectric Point: 4.7003

>T160097 WP_012117368.1 NZ_CP054714:c1226078-1225332 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQADKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T160097 NZ_CP070292:2535759-2536037 [Escherichia albertii]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCTGCAAGCAGTCTTGTTGATATCGATATGCCCGGTTACCGACTAC
ACCCATTAAGCGGCGATCGCGATGGCATCTGGGCAATATCTGTATCAGGCAACTGGCGAATCACGTTTGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT160097 WP_003154807.1 NZ_CP054714:c1225331-1225161 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT160097 NZ_CP070292:2536037-2536321 [Escherichia albertii]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGACTGTTGACCGGAAAAGCGGCTTTGACGCCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCTGAG
AAAACGGTGGATGTGTCGCGGTTGCGCCGTCTGGTGACGCAATAA

Structures

Antitoxin

• AlphaFold DB: I2HQ14