160096

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 488650..489287 Replicon chromosome
Accession NZ_CP054714
Organism Bacillus velezensis strain KKLW

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HUO02_RS02490 Protein ID WP_003156187.1
Coordinates 488937..489287 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag HUO02_RS02485 Protein ID WP_003156188.1
Coordinates 488650..488931 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUO02_RS02465 485015..485614 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
HUO02_RS02470 485707..486072 + 366 WP_012116869.1 holo-ACP synthase -
HUO02_RS02475 486237..487244 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
HUO02_RS02480 487361..488530 + 1170 WP_012116870.1 alanine racemase -
HUO02_RS02485 488650..488931 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HUO02_RS02490 488937..489287 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HUO02_RS02495 489405..490226 + 822 WP_003156182.1 STAS domain-containing protein -
HUO02_RS02500 490231..490596 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
HUO02_RS02505 490599..491000 + 402 WP_003156178.1 anti-sigma regulatory factor -
HUO02_RS02510 491012..492019 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
HUO02_RS02515 492083..492412 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
HUO02_RS02520 492409..492891 + 483 WP_007609591.1 anti-sigma B factor RsbW -
HUO02_RS02525 492857..493645 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
HUO02_RS02530 493645..494247 + 603 WP_012116871.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T160096 WP_003156187.1 NZ_CP054714:488937-489287 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T160096 NZ_CP070292:2511797-2512183 [Escherichia albertii]
ATGATTTGGGTCAGTGCACAGGAGGTCATTGCCTTCCACGATCGAATTTTGCAACGTTTTCCCGGTGTGGCGGGCCTGAC
TGACCCCGGCAGAGCACAGGCGTTGATTTATCGCGTGCAGAATCGTGTTCACTACGAGGGCGTTACTGACCTGTTTGAAC
TGGCAGCAACCTACTGGGTTGCCATCGCTCGCGGTCACATCTTTCATGATGGTAATAAACGTACCGCATTTTTCGTTACT
ATGACCTTTCTTTGGCGCAATGGCATCCGTATCCGTGACGTTGATAACTCGCTGGAGAATCTGACTGTCGAAGCGGCTAC
CGGAGAAAAAACGGTCGGTCAATTAGCGCGATGTTTACGTGAACGAGTGGATAGTTCCGGGAATTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT160096 WP_003156188.1 NZ_CP054714:488650-488931 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT160096 NZ_CP070292:2511579-2511800 [Escherichia albertii]
ATGCAAACTTACACTTCCACACAAGCCAGAGCCAATATTGCCACTGTACTGGATACCGCGTTGAATGGTGAGCCAGTTGA
AATCACCCGTCGCGACGGCAGCTCTGCCGTGCTCATTAGTAAAGCCGCATTCGAGGCGTATCAAAATGCGAAACTGGACG
CGGAATTTGATGCCATCATGCAACGTCATGAACACACAATTGAAGCGTTGACTGATCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References