Detailed information of TA system TA160077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2343064..2343613
• Replicon: chromosome
• Accession: NZ_CP054698
• Organism: Nostoc edaphicum CCNP1411

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HUN01_RS12275
• Protein ID: WP_181931505.1
• Coordinates: 2343064..2343402 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HUN01_RS12280
• Protein ID: WP_181931506.1
• Coordinates: 2343389..2343613 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUN01_RS12245	2339389..2339556	+	168	WP_181931501.1	hypothetical protein	-
HUN01_RS12250	2339719..2340387	-	669	WP_069074098.1	DUF1345 domain-containing protein	-
HUN01_RS12255	2340637..2341203	+	567	WP_181931502.1	thermonuclease family protein	-
HUN01_RS12260	2341260..2342141	-	882	WP_069074093.1	sugar ABC transporter permease	-
HUN01_RS12265	2342286..2342771	-	486	WP_181931503.1	DUF3368 domain-containing protein	-
HUN01_RS12270	2342776..2343015	-	240	WP_203219554.1	hypothetical protein	-
HUN01_RS12275	2343064..2343402	-	339	WP_181931505.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUN01_RS12280	2343389..2343613	-	225	WP_181931506.1	ribbon-helix-helix domain-containing protein	Antitoxin
HUN01_RS12285	2343750..2344026	+	277	Protein_1898	BrnT family toxin	-
HUN01_RS12290	2343989..2344264	+	276	WP_181931507.1	BrnA antitoxin family protein	-
HUN01_RS12295	2344521..2344730	-	210	WP_181931508.1	hypothetical protein	-
HUN01_RS12300	2344720..2345163	-	444	Protein_1901	PIN domain-containing protein	-
HUN01_RS12305	2345293..2346798	-	1506	WP_181932666.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
HUN01_RS12310	2347258..2348286	-	1029	Protein_1903	caspase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2335341..2361402	26061

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12265.38 Da
• Isoelectric Point: 9.9502

>T160077 WP_181931505.1 NZ_CP054698:c2343402-2343064 [Nostoc edaphicum CCNP1411]
MKRGDIYYANLSPVMGSEMGKRRPILIVSNDISNSAATTVTILPLTSNVNRVYPFEVLLNPEISGLTKPSKVQAQQVRTI
SKQRITGEVLGSLNEEMMVLIDAALKLHLGLV

Nucleotide

• Length: 339 bp

>T160077 NZ_CP070290:c593811-593347 [Escherichia albertii]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAACCCATCATCATCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACACTGCGCACCTACGGTAAAAAAACAGATGCCTATACAGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCAAGAGACAGAAGAAAAGCATTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8486.44 Da
• Isoelectric Point: 4.1717

>AT160077 WP_181931506.1 NZ_CP054698:c2343613-2343389 [Nostoc edaphicum CCNP1411]
MNVEKLSISLPPSLVEFVENYKRNKGCKSRSQVIEEALELLRNRELEAAYREASAEVDNDWDVTIADGLTDETW

Nucleotide

• Length: 225 bp

>AT160077 NZ_CP070290:c594146-593811 [Escherichia albertii]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA