Detailed information of TA system TA160057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2817820..2819257
• Replicon: chromosome
• Accession: NZ_CP054665
• Organism: Escherichia coli strain IGC_EcoliInv_1.1

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: HUR94_RS13965
• Protein ID: WP_000155570.1
• Coordinates: 2817820..2818893 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: HUR94_RS13970
• Protein ID: WP_000461704.1
• Coordinates: 2818886..2819257 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR94_RS13950 (2813253)	2813253..2815442	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
HUR94_RS13955 (2815439)	2815439..2817055	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
HUR94_RS13960 (2817415)	2817415..2817678	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
HUR94_RS13965 (2817820)	2817820..2818893	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
HUR94_RS13970 (2818886)	2818886..2819257	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
HUR94_RS13975 (2819612)	2819612..2820475	+	864	WP_000787597.1	GTPase family protein	-
HUR94_RS13980 (2820567)	2820567..2821388	+	822	WP_000197391.1	DUF932 domain-containing protein	-
HUR94_RS13985 (2821605)	2821605..2822306	+	702	WP_000189409.1	WYL domain-containing protein	-
HUR94_RS13990 (2822347)	2822347..2822583	+	237	WP_001144029.1	protein YpjK	-
HUR94_RS13995 (2822583)	2822583..2823026	+	444	WP_000824222.1	lipoprotein YfjS	-
HUR94_RS14000 (2823050)	2823050..2823517	+	468	WP_000702495.1	protein YfjT	-
HUR94_RS14005 (2823904)	2823904..2824056	-	153	Protein_2735	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2761047..2819257	58210

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T160057 WP_000155570.1 NZ_CP054665:2817820-2818893 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T160057 NZ_CP070286:151855-152142 [Escherichia albertii]
ATGACTTACACAGTCAAATTCAGGGATGATGCACTGAAAGAGTGGCTGAAGCTGGATAAGGCCATTCAACAGCAGTTTGC
GAAAAAGCTAAAGAAGTGCAGTGAAAATCCTCATATTCCCTCAGCTAAGCTGCGCGGGATAAAAGACTGCTACAAAATCA
AATTACGTGCGTCTGGTTTTCGTCTGGTCTATCAGGTGATTGATGATCAATTAATTATCGCTGTTGTTGCTGTAGGGAAA
CGTGAACGTAGTGATGTTTATCAACTTGCCAGCGAACGAATGAGGTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT160057 WP_000461704.1 NZ_CP054665:2818886-2819257 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT160057 NZ_CP070286:151607-151858 [Escherichia albertii]
ATGCCTAATATCATTCTTACCGACACTAGCGCCAGCGTAAGCGAGCTTAAGAAAAACCCGATGGCTACAGTCAGCGCCGG
TGATGGCTACCCGGTTGCTATTCTGAACCGCAACCAGCCTGCCTTTTACTGTGTGCCTGCAGATCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAACTGGTTAAACTGGTTGCCGAACGCAGCAACCAACAGCTGCACGACGTTGATCTGGAT
AGCTACTTATGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• AlphaFold DB: A0A373F4G4