Detailed information of TA system TA160044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1514103..1514741
• Replicon: chromosome
• Accession: NZ_CP054665
• Organism: Escherichia coli strain IGC_EcoliInv_1.1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HUR94_RS07540
• Protein ID: WP_000813794.1
• Coordinates: 1514103..1514279 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUR94_RS07545
• Protein ID: WP_001270286.1
• Coordinates: 1514325..1514741 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR94_RS07520 (1509722)	1509722..1510936	-	1215	WP_001326689.1	BenE family transporter YdcO	-
HUR94_RS07525 (1510989)	1510989..1511525	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HUR94_RS07530 (1511598)	1511598..1513559	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUR94_RS07535 (1513651)	1513651..1513881	-	231	WP_000494244.1	YncJ family protein	-
HUR94_RS07540 (1514103)	1514103..1514279	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUR94_RS07545 (1514325)	1514325..1514741	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUR94_RS07550 (1514820)	1514820..1516226	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HUR94_RS07555 (1516471)	1516471..1517616	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HUR94_RS07560 (1517634)	1517634..1518647	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
HUR94_RS07565 (1518648)	1518648..1519589	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T160044 WP_000813794.1 NZ_CP054665:1514103-1514279 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T160044 NZ_CP070284:1612450-1612836 [Escherichia albertii]
ATGATTTGGGTCAGTGCACAGGAGGTCATTGCCTTCCACGATCGAATTTTGCAACGTTTTCCCGGTGTGGCGGGCCTGAC
TGACCCCGGCAGAGCACAGGCGTTGATTTATCGCGTGCAGAATCGTGTTCACTACGAGGGCGTTACTGACCTGTTTGAAC
TGGCAGCAACCTACTGGGTTGCCATCGCTCGCGGTCACATCTTTCATGATGGTAATAAACGTACCGCATTTTTCGTTACT
ATGACCTTTCTTTGGCGCAATGGCATCCGTATCCGTGACGTTGATAACTCGCTGGAGAACCTGACTGTCGAAGCGGCTAC
CGGAGAAAAAACGGTCGGTCAATTAGCGCGATGTTTACGTGAACGAGTGGATAGTTCCGGGAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT160044 WP_001270286.1 NZ_CP054665:1514325-1514741 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT160044 NZ_CP070284:1612232-1612453 [Escherichia albertii]
ATGCAAACTTACACTTCCACACAAGCCAGAGCCAATATTGCCACTGTACTGGATACCGCGTTGAATGGTGAGCCAGTTGA
AATCACCCGTCGCGACGGCAGCTCAGCCGTGCTCATTAGTAAAGCCGCATTCGAGGCGTATCAAAATGCGAAACTGGACG
CGGAATTTAATGCCATCATGCAACGTCATGAACACACAATTGAAGCGTTGACTGATCGATGA

Structures

Toxin

• PDB: 6HPB