Detailed information of TA system TA160032

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 262551..263230
• Replicon: chromosome
• Accession: NZ_CP054665
• Organism: Escherichia coli strain IGC_EcoliInv_1.1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: HUR94_RS01245
• Protein ID: WP_000854672.1
• Coordinates: 262551..262892 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: HUR94_RS01250
• Protein ID: WP_000070395.1
• Coordinates: 262913..263230 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR94_RS01220 (257828)	257828..258229	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
HUR94_RS01225 (258268)	258268..259323	-	1056	WP_000749863.1	phosphoporin PhoE	-
HUR94_RS01230 (259611)	259611..260714	+	1104	WP_001285288.1	glutamate 5-kinase	-
HUR94_RS01235 (260726)	260726..261979	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
HUR94_RS01245 (262551)	262551..262892	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HUR94_RS01250 (262913)	262913..263230	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HUR94_RS01255 (263249)	263249..263470	-	222	WP_000691994.1	DUF987 domain-containing protein	-
HUR94_RS01260 (263479)	263479..263955	-	477	WP_000811693.1	RadC family protein	-
HUR94_RS01265 (263971)	263971..264429	-	459	WP_000211838.1	antirestriction protein	-
HUR94_RS01270 (264527)	264527..264766	-	240	WP_000194654.1	DUF905 family protein	-
HUR94_RS01275 (264843)	264843..265310	-	468	WP_001547765.1	protein YkfB	-
HUR94_RS01280 (265333)	265333..265776	-	444	WP_000824223.1	lipoprotein YafY	-
HUR94_RS01285 (265776)	265776..265952	-	177	WP_001285112.1	hypothetical protein	-
HUR94_RS01290 (265999)	265999..266190	-	192	Protein_249	DeoR family transcriptional regulator	-
HUR94_RS01295 (266407)	266407..267228	-	822	WP_000197389.1	DUF932 domain-containing protein	-
HUR94_RS01300 (267320)	267320..268183	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T160032 WP_000854672.1 NZ_CP054665:c262892-262551 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T160032 NZ_CP070282:58870-59268 [Escherichia albertii]
ATGCTGAAATTCATGCTGGACACGAATATCTGTATCTTCACGATTAAGAACAAACCGTCGCATATTCGGGAACGATTCAA
TCTGAACTCGGGGCGAATGTGCATCAGCTCAGTGACCGTGATGGAGCTGGTATACGGTGCTGAAAAAAGTCAGTTTCCGG
AGCGTAATCTCGCGATTATCGAAGGATTTATTTCGCGCCTTGACGTACTGGATTACGACTACGCTGCTGCCACACATACC
GGTCAGATCCGTGCTGAGCTCGCCCGTCAGGGACGTCCGGTTGGGCCGTTTGATCAGATGATTGCCGGTCATGCCCGCAG
CCGGGGACTCATTGTGGTCACAAACAATACCCGGGAGTTTGAACGTATCGCCGGCCTTCGTCTGGAAGACTGGTGTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT160032 WP_000070395.1 NZ_CP054665:c263230-262913 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT160032 NZ_CP070282:58643-58870 [Escherichia albertii]
ATGGAAACGACCGTATTTCTCAGTAACCGGAGTCAGGCAGTCAGGCTGCCGAAGGCTGTAGCCCTGCCTGAAGACGTAAA
AAAAGTCGACGTTATTGCTGTTGGCCGTACCCGGATCATTACCCCGGCAGGAGAGTCCTGGGACAGCTGGTTCGATGGCG
ACAGTGTCAGCGCAGATTTCATGAATGACAGAGAACAACCCGCCGTACAGGAAAGGGAGTCGTTCTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684